Product Information

Contents: Affinity Purified anti-human NKG2D / CD314 (activating)
Catalog Number: 14-5879
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 5C6
Isotype: Mouse IgG2a

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (open histogram) or 0.25 μg of Purified 5C6 (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5879	Affinity Purified anti-human NKG2D / CD314 (activating)	N/A	N/A	FA FC IHC IP
16-5879	Functional Grade* Purified anti-human NKG2D / CD314 (activating)	N/A	N/A	FA FC
13-5879	Biotin anti-human NKG2D / CD314 (activating)	N/A	N/A	FC
12-5879	Phycoerythrin (PE) anti-human NKG2D / CD314 (activating)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 5C6 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, γδ T cells, some CD4⁺ and CD8⁺ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to γδ TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 5C6 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been reported in inhibition of ligand binding in in vitro assays. Please use Functional Grade purified 5C6 in functional assays.)

Applications Tested

The 5C6 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Veronika Groh, Alexander Steinle, Stefan Bauer, and Thomas Spies. 1998. Recognition of Stress-Induced MHC Molecules by Intestinal Epithelial T Cells. Science. 279:1737-1740.

Stefan Bauer, Veronika Groh, Jun Wu, Alexander Steinle, Joseph H. Phillips, Lewis L. Lanier, and Thomas Spies. 1999. Activation of NK Cells and T Cells by NKG2D, a Receptor for Stress-Inducible MICA. Science. 285: 727-729.

Seiler M, Brabcova I, Viklicky O, Hribova P, Rosenberger C, Pratschke J, Lodererova A, Matz M, Schönemann C, Reinke P, Volk HD, Kotsch K. Heightened expression of the cytotoxicity receptor NKG2D correlates with acute and chronic nephropathy after kidney transplantation. Am J Transplant. 2007 Feb;7(2). (5c6, IH/F, PubMed)

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4724  Affinity Purified Mouse IgG2a, K Isotype Control
Cat. 14-5878  Affinity Purified anti-human NKG2D / CD314 (activating) (clone 1D11)