Product Information

Contents: Affinity Purified anti-mouse GITR (TNFRSF18)
Catalog Number: 14-5874
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: DTA-1
Isotype: Rat IgG2b

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-5874-80	25 ug	$85
14-5874-82	100 ug	$255

*International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with .015 μg of Purified Rat IgG2b Isotype Control (cat. 14-4031) (open histogram) or .015 μg of Purified anti-mouse GITR (DTA-1) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5874	Fluorescein isothiocyanate (FITC) anti-mouse GITR (TNFRSF18, AITR)	488	518	FC
12-5874	Phycoerythrin (PE) anti-mouse GITR (TNFRSF18, AITR)	488	575	FC
13-5874	Biotin anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FC
14-5874	Affinity Purified anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FA FC IP
16-5874	Functional Grade* Purified anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FA FC
17-5874	Allophycocyanin (APC) anti-mouse GITR (TNFRSF18, AITR)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naïve mice, GITR is expressed predominantly by CD4⁺CD25⁺ T regulatory cells (Treg) and by CD25⁺ CD4⁺ CD8^- thymocytes. Stimulation of GITR abrogates Treg cell-mediated suppression. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.

Usage

For research use only, not for diagnostic or therapeutic use. The DTA-1 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported for in vitro and in vivo blocking of mouse GITR. (Please use Functional Grade purified DTA-1 in functional assays.)

Applications Tested

This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.015 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 14-4031    Purified Rat IgG2b Isotype Control
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 11-4811    FITC Anti-Rat IgG
Cat. 13-4813    Biotin Anti-Rat IgG (clone Polyclonal)
Cat. 71-5775    FITC anti-mouse/rat Foxp3 Staining Set (clone FJK-16s Set)
Cat. 72-5775    PE anti-mouse/rat Foxp3 Staining Set (clone FJK-16s Set)

References

Shimizu J, Yamazaki S, Takahashi T, Ishida Y, Sakaguchi S. Stimulation of CD25(+)CD4(+) regulatory T cells through GITR breaks immunological self-tolerance. Nat Immunol. 2002 Feb;3(2):135-42.