Product Information

Contents: Affinity Purified anti-human Nod2
Catalog Number: 14-5869
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 2D9
Isotype: Mouse IgG1, κ

Immunoblotting of 293T cells transfected with FLAG-NOD2 (Lane A) or vector alone (Lane B); probed with 2 ug/mL purified 2D9 and revealed with 1:1000 dilution of HRP rat anti-mouse IgG. NOD2 is detected at ~110 kDa.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5869	Affinity Purified anti-human Nod2	N/A	N/A	IHC IP WB

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Description

The 2D9 monoclonal antibody reacts with NOD2 protein, also known as caspase recruitment domain-containing protein 15 (CARD-15), member of a family of intracellular proteins that contain an N-terminal caspase recruitment domain (CARD), a nucleotide-binding domain (NBD), and a C-terminal regulatory domain. A NOD2 variant has been identified and termed NOD2B. RT-PCR analysis revealed expression primarily in peripheral monocytes. Expression of NOD2 or NOD2B resulted in NFKB activation, and mutants lacking the LRRs had enhanced NFKB activation. Both intact CARD domains are necessary and sufficient for IKK-gamma and RICK -dependent NFKB activation. Coimmunoprecipitation analysis showed that the CARD domain of RICK interacts with the CARD domains of NOD2. It has been proposed that NOD2 serves as an intracellular receptor for bacterial products in monocytes and transduces signals leading to NFKB activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 2D9 antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

The 2D9 antibody has been tested by immunoblotting (WB) of lysates from 293T cells transfected with vector alone or FLAG-Nod2 (2ug/ml).

References

Ogura Y, Lala S, Xin W, Smith E, Dowds TA, Chen FF, Zimmermann E, Tretiakova M, Cho JH, Hart J, Greenson JK, Keshay S, Nunez G. 2003. Expression of NOD2 in Paneth cells: a possible link to Crohn’s ileitis. Gut. 52(11):1591-7.

Related Products

Cat. 18-8877 See Catalog # 18-8817 for an alternative product: Mouse TrueBlot™: Horseradish Peroxidase (HRP) anti-mouse IgG