Product Information

Contents: Affinity Purified anti-mouse/human integrin β7
Catalog Number: 14-5867
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: FIB504
Isotype: Rat IgG2a, κ

Staining of normal human peripheral blood cells with 0.06 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.06 μg of Purified anti-mouse/human integrin β7 (FIB504) (colored histogram) followed by PE Anti-Rat IgG (cat. 12-4822). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5867	Affinity Purified anti-mouse/human integrin beta7	N/A	N/A	FA FC
13-5867	Biotin anti-mouse/human integrin beta7	N/A	N/A	FC
11-5867	Fluorescein isothiocyanate (FITC) anti-mouse/human integrin beta7	488	518	FC
12-5867	Phycoerythrin (PE) anti-mouse/human integrin beta7	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The FIB504 monoclonal antibody reacts with human and mouse integrin β7. Integrin β7 is a 130 kD glycoprotein which associates with integrin α4 (CD49d) to form the α4β7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with αE (CD103) to form the αEβ7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Main ligands for integrin α4β7 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the main ligand of integrin αEβ7 is E-cadherin (CD324). Integrin β7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response. The FIB504 antibody has been reported to have blocking activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This FIB504 antibody has been reported for use in flow cytometric analysis and functional assays.

Applications Tested

This FIB504 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Rodriguez MW, Paquet AC, Yang YH, Erle DJ. Differential gene expression by integrin β7+ and β7- memory T helper cells. BMC Immunol. 2004 Jul 5;5:13. (FIB504, FC, PubMed)

Meerschaert J, Vrtis RF, Shikama Y, Sedgwick JB, Busse WW, Mosher DF. Engagement of α4β7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro. J Immunol. 1999 Dec 1;163(11):6217-27. (FIB504, FA, PubMed)

Andrew DP, Berlin C, Honda S, Yoshino T, Hamann A, Holzmann B, Kilshaw PJ, Butcher EC. Distinct but overlapping epitopes are involved in α4β7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol. 1994 Nov 1;153(9):3847-61. (FIB504, FA, PubMed)

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