Product Information

Contents: Affinity Purified anti-mouse Nod2
Catalog Number: 14-5858
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 26mNOD2
Isotype: Rat IgG1, k

Immunoblot of 293T cell control lysate (1) and 293T cells transfected with mouse NOD2 (2). 2 x10⁵ cells were loaded per lane, resolved by SDS-PAGE and transferred to PVDF. The blots were then probed with 2µg/ml of 26mNOD2 antibody followed by polyclonal anti-rat secondary antibody conjugated to HRP and visualized by chemiluminescence.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5858	Affinity Purified anti-mouse Nod2	N/A	N/A	WB

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Description

The monoclonal antibody 26mNOD2 reacts with mouse NOD2. NOD2, also referred to as caspase recruitment domain-containing protein 15 (CARD 15), is a cytosolic protein of approximately 115kDa. NOD2 contains a central NOD (nucleotide oligomerization domain) that is flanked by a C-terminal leucine-rich repeat (LRR) domain and an N-terminal CARD domain. The LRR confers recognition of microbial components such as muramyl dipeptide and the CARD domain confers the ability to interact with RIP2 via homotypic interaction with the RIP2 CARD. This interaction leads to downstream activation of NF-κB.

The antibody 26mNOD2 has been shown to recognize protein from transgenic and transfected cells. Expression on endogenous tissue is still under evaluation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 26mNOD2 antibody has been reported for use in immunoblotting (WB).

Applications Tested

This 26mNOD2 antibody has been tested by immunoblotting of lysates of 293T cells transfected with mouse NOD2.

Special Notes

This antibody has been validated on transfected cell lines and cells from NOD2-transgenic mice.

References

Yang Z, Fuss IJ, Watanabe T, Asano N, Davey MP, Rosenbaum JT, Strober W, Kitani A. NOD2 transgenic mice exhibit enhanced MDP-mediated down-regulation of TLR2 responses and resistance to colitis induction. Gastroenterology. 2007 Nov;133(5):1510-21. (26mNOD2, WB)

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