Product Information

Contents: Affinity Purified anti-mouse ESAM (Endothelial cell-Selective Adhesion Molecule)
Catalog Number: 14-5852
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 1G8
Isotype: Rat IgG2a, κ

Staining of bEnd.3 mouse endothelioma cells with 0.06 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.06 μg of Purified anti-mouse ESAM (1G8) (colored histogram) followed by PE Anti-Rat IgG (cat. 12-4822). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5852	Affinity Purified anti- mouse ESAM (Endothelial cell-Selective Adhesion Molecule)	N/A	N/A	FC IH/F IP
11-5852	Fluorescein isothiocyanate (FITC) anti-mouse ESAM (Endothelial cell-Selective Adhesion Molecule)	488	518	FC
12-5852	Phycoerythrin (PE) anti-mouse ESAM (Endothelial cell-Selective Adhesion Molecule)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 1G8 monoclonal antibody reacts with mouse ESAM (endothelial cell-selective adhesion molecule). ESAM is a 55-kDa membrane protein composed of 2 extracellular Ig domains, a single transmembrane domain, and a 120 amino acid cytoplasmic domain. ESAM is related to members of the junctional adhesion molecule (JAM) family, and is expressed on endothelial cells and activated platelets. ESAM at endothelial tight junctions plays a role in the regulation of vascular permeability, in response to agents such as VEGF or extravasating neutrophils.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 1G8 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.

Applications Tested

This 1G8 antibody has been tested by flow cytometric analysis of the bEnd.3 mouse endothelioma cell line. This can be used at less than or equal to 0.125 µg per 1 million cells in 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nasdala I, Wolburg-Buchholz K, Wolburg H, Kuhn A, Ebnet K, Brachtendorf G, Samulowitz U, Kuster B, Engelhardt B, Vestweber D, Butz S. A transmembrane tight junction protein selectively expressed on endothelial cells and platelets. J Biol Chem. 2002 May 3;277(18):16294-303. Epub 2002 Feb 14. (1G8, FC, IP, IH/F PubMed)

Wegmann F, Petri B, Khandoga AG, Moser C, Khandoga A, Volkery S, Li H, Nasdala I, Brandau O, Fassler R, Butz S, Krombach F, Vestweber D. ESAM supports neutrophil extravasation, activation of Rho, and VEGF-induced vascular permeability. J Exp Med. 2006 Jul 10;203(7):1671-7. Epub 2006 Jul 3. (PubMed)

Wegmann F, Ebnet K, Du Pasquier L, Vestweber D, Butz S. Endothelial adhesion molecule ESAM binds directly to the multidomain adaptor MAGI-1 and recruits it to cell contacts. Exp Cell Res. 2004 Oct 15;300(1):121-33. (PubMed)

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