Affinity Purified anti-human BAMBI (BMP and activin membrane-bound inhibitor, TGF-beta pseudoreceptor)

RUO: For Research Use Only

Contents: Affinity Purified anti-human BAMBI (BMP and activin membrane-bound inhibitor, TGF-beta pseudoreceptor)
Catalog Number: 14-5826
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Clone: 4E8
Host/Isotype: Mouse IgG1
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
14-5826-80	25 ug	To view relevant pricing, please select your country and resume browsing.
14-5826-82	100 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4011	FITC Anti-Mouse IgG
11-4317	Streptavidin-FITC (Fluorescein isothiocyanate)
12-4317	Streptavidin-PE (Phycoerythrin)
13-4013	Biotin Anti-Mouse IgG (Polyclonal)
14-4714	Affinity Purified Mouse IgG1, K Isotype Control
17-4317	Streptavidin Allophycocyanin (SA-APC)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
12-5826	Phycoerythrin (PE) anti-human BAMBI (BMP and activin membrane-bound inhibitor, TGF-beta pseudoreceptor)	488	575	FC	RUO
13-5826	Biotin anti-human BAMBI (BMP and activin membrane-bound inhibitor, TGF-beta pseudoreceptor)	N/A	N/A	FC	RUO
14-5826	Affinity Purified anti-human BAMBI (BMP and activin membrane-bound inhibitor, TGF-beta pseudoreceptor)	N/A	N/A	FC	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The 4E8 antibody reacts with BAMBI (BMP and activin membrane-bound inhibitor). TGF-beta signals through a heteromeric cell-surface complex of two types of transmembrane serine/threonine kinases, the type I (TRI) and type II (TRII) receptors. TRII activates TRI, which, in turn, propagates the TGF-beta signal by phosphorylating the Smads, Smad2 and Smad3. BAMBI is a transmembrane protein that lacks an intracellular kinase domain but has sequence similarity to the extracellular domain of TRI and thereby inhibits TGF-beta signaling by forming a heterodimer with TRII. BAMBI expression is aberrantly elevated in most colorectal and hepatocellular carcinomas, compared with the corresponding non-cancerous tissues. Beta-catenin activates transcription of BAMBI, suggesting a possible mechanism by which tumor cells escape the growth inhibitory activities of TGF-beta.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 4E8 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 4E8 antibody has been tested by flow cytometric analysis of human BAMBI transfected 293 cells. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Takashi, S., et al. 2004. Identification of BMP and Activin Membrane-bound Inhibitor (BAMBI), an Inhibitor of Transforming Growth Factor- Signaling, as a Target of the beta -Catenin Pathway in Colorectal Tumor Cells. J. Biol. Chem. 279: 6840-6846.