Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 4E8 antibody reacts with BAMBI (BMP and activin membrane-bound inhibitor).
TGF-beta signals through a heteromeric cell-surface complex of two types of transmembrane serine/threonine kinases, the type I (TRI) and type II (TRII) receptors. TRII activates TRI, which, in turn, propagates the TGF-beta signal by phosphorylating the Smads, Smad2 and Smad3. BAMBI is a transmembrane protein that lacks an intracellular kinase domain but has sequence similarity to the extracellular domain of TRI and thereby inhibits TGF-beta signaling by forming a heterodimer with TRII. BAMBI expression is aberrantly elevated in most colorectal and hepatocellular carcinomas, compared with the corresponding non-cancerous tissues. Beta-catenin activates transcription of BAMBI, suggesting a possible mechanism by which tumor cells escape the growth inhibitory activities of TGF-beta.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 4E8 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 4E8 antibody has been tested by flow cytometric analysis of human BAMBI transfected 293 cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Takashi, S., et al. 2004. Identification of BMP and Activin Membrane-bound Inhibitor (BAMBI), an Inhibitor of Transforming Growth Factor- Signaling, as a Target of the beta -Catenin Pathway in Colorectal Tumor Cells. J. Biol. Chem. 279: 6840-6846.
