Product Information

Contents: Affinity Purified anti-mouse/human T-bet (Tbet, Th1-specific T box transcription factor)
Catalog Number: 14-5825
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio4B10 (4B10, 4-B10)
Isotype: Mouse IgG1

CD4+ T cells were sorted from mouse spleen, activated with anti-mouse CD3 and anti-mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-activated (right) cells were probed with purified eBio4B10 at 2 μg/ml, and revealed with HRP anti-mouse IgG.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5825	Affinity Purified anti-mouse/human T-Bet (Tbet, Th1-specific T box transcription factor)	N/A	N/A	FC IP WB
12-5825	Phycoerythrin (PE) anti-mouse/human T-Bet (Tbet, Th1-specific T box transcription factor)	488	575	IC Flow
51-5825	Alexa Fluor® 647 anti-mouse/human T-Bet (Tbet, Th1-specific T box transcription factor)	633	668	IC Flow
45-5825	PerCP-Cy5.5 anti-mouse/human T-Bet (Tbet, Th1-specific T box transcription factor) (PE-Cy5.5 replacement)	488	690	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-γ, and T-Bet also regulates the expression of IFN-γ, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-γ, T-Bet is also known to regulate the expression of IL-12Rβ and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio4B10 (4B10, 4-B10) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).

Applications Tested

This eBio4B10 (4B10, 4-B10) antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 μg/ml as a starting dilution for western blotting.

References

Szabo SJ, Kim ST, Costa GL, Zhang X, Fathman CG, Glimcher LH. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell. 2000 Mar 17;100(6):655-69. (4B10, FC, WB, PubMed)

Usui T, Preiss JC, Kanno Y, Yao ZJ, Bream JH, O'Shea JJ, Strober W. T-bet regulates Th1 responses through essential effects on GATA-3 function rather than on IFNG gene acetylation and transcription. J Exp Med. 2006 Mar 20;203(3):755-66.

Hwang ES, Hong JH, Glimcher LH. IL-2 production in developing Th1 cells is regulated by heterodimerization of RelA and T-bet and requires T-bet serine residue 508. J Exp Med. 2005 Nov 7;202(9):1289-300.

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