Product Information

Contents: Affinity Purified anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)
Catalog Number: 14-5821
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: Avas12a1
Isotype: Rat IgG2a, κ

Surface staining of bEnd.3 cells with anti-mouse Flk-1 (Avas12a1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5821	Affinity Purified anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	N/A	N/A	FC IHC WB
13-5821	Biotin anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	N/A	N/A	FC
12-5821	PE anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	488	575	FC
17-5821	Allophycocyanin (APC) anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	633	660	FC
56-5821	Alexa Fluor® 700 anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) (APC-Cy5.5 Equivalent)	633	723	FC
57-5821	Pacific Blue® anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The Avas12a1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

This Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kataoka, H., N. Takakura, et al. (1997)."Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells." Dev Growth Differ 39(6): 729-40.

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