Product Information

Contents: Affinity Purified anti-mouse V beta 8.1, 8.2 TCR (Vβ8.1, 8.2-TCR, Vβ-8.1, 8.2 TCR)
Catalog Number: 14-5813
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: KJ16 (KJ-16, KJI6, KJ-I6)
Isotype: Rat IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5813	Affinity Purified anti-mouse V beta 8.1, 8.2 TCR (Vb8.1, 8.2-TCR, Vb-8.1, 8.2 TCR)	N/A	N/A	FC IP
13-5813	Biotin anti-mouse V beta 8.1, 8.2 TCR (Vb8.1, 8.2-TCR, Vb-8.1, 8.2 TCR)	N/A	N/A	FC IP
11-5813	Fluorescein isothiocyanate (FITC) anti-mouse V beta 8.1, 8.2 TCR (Vb8.1, 8.2-TCR, Vb-8.1, 8.2 TCR)	488	518	FC
12-5813	Phycoerythrin (PE) anti-mouse V beta 8.1, 8.2 TCR (Vb8.1, 8.2-TCR, Vb-8.1, 8.2 TCR)	488	575	FC
15-5813	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse V beta 8.1, 8.2 TCR (Vb8.1, 8.2-TCR, Vb-8.1, 8.2 TCR)	488	670	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The KJ16 monoclonal antibody recognizes mouse T cell receptors (TCR) containing Vβ 8.1 or 8.2, which is expressed by 15- 20% of peripheral T cells in most strains of mice (ie. Balb/c, AKR, C57BL/6, B10.A) and by a number of T cell hybridomas (ie. DO- 11.10, 3DT-52.5, D1G10G11, SKK-9.11, SKK-45.10, MDK16, S18.4).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This KJ16 (KJ-16, KJI6, KJ-I6) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested

This KJ16 (KJ-16, KJI6, KJ-I6) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Haskins, K., C. Hannum, J. White , N. Roehm, R. Kubo, J. Kappler, and P. Marrack. 1984. The antigen-specific, major histocompatibility complex-restricted receptor on T cells. J. Exp. Med. 160: 452-471.

Yagüe, J., J. White, C. Coleclongh, J. Kappler, E. Palmer and P. Marrack. 1985. The T cell receptor: the γ and ß chains define idiotype, and antigen and MHC specificity. Cell 42: 81-87.

Sim, G. K., A. A. Augstin. 1985. Vß gene polymorhism and a major polyclonal T cell receptor idiotype. Cell 42: 89-92.

Related Products

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Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)