Product Information

Contents: Affinity Purified anti-mouse V alpha 2 TCR (Vα2-TCR, Vα-2 TCR)
Catalog Number: 14-5812
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: B20.1
Isotype: Rat IgG2a

Staining of C57Bl/6 lymph node cells with 0.125 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.125 μg of Purified anti-mouse Va-2 TCR (B20.1) (colored histogram) followed by PE Anti-Rat IgG (cat. 12-4822). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5812	Affinity Purified anti-mouse V alpha 2 TCR (Va2-TCR, Va-2 TCR)	N/A	N/A	FC IP
13-5812	Biotin anti-mouse V alpha 2 TCR (Va2-TCR, Va-2 TCR)	N/A	N/A	FC IP
11-5812	Fluorescein isothiocyanate (FITC) anti-mouse V alpha 2 TCR (Va2-TCR, Va-2 TCR)	488	518	FC
12-5812	Phycoerythrin (PE) anti-mouse V alpha 2 TCR (Va2-TCR, Va-2 TCR)	488	575	FC
17-5812	Allophycocyanin (APC) anti-mouse V alpha 2 TCR (Va2-TCR, Va-2 TCR)	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) Vα-2 chains. This antibody recognizes the majority of the TCR Vα-2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, Vδ8 due to the high degree of homology.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This B20.1 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested

This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended to preblock Fc receptors for 10 minutes using 0.5 µg of purified anti-mouse CD16/32 (cat. 14-0161). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Pircher, H., N. Rebai, M. Groettrup, C. Gregoire, D. E. Speiser, M. P. Happ, E. Palmer, R. M. Zinkernagel, H. Hengartner, B. Malissen. 1992. Eur. J. Immunol. 22: 399-404.

Gregoire, C., N. Rebai, F. Schweisguth, A. Necker, G. Mazza, N. Auphan, A. Millward, Anne-Marie Schmitt-Verhulst, B. Malissen. 1991. Proc. Natl. Acad. Sci. USA. 88: 8077-8081.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321  Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)