Product Information

Contents: Affinity Purified anti-mouse Ly-49A/D (Ly49A/D)
Catalog Number: 14-5783
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio12A8 (12A8)
Isotype: Rat IgG2a, κ

Staining of 3-day IL-2 activated with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-mouse Ly-49A/D (12A8) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5783	Affinity Purified anti-mouse Ly-49A/D (Ly49A/D)	N/A	N/A	FC IP
12-5783	Phycoerythrin (PE) anti-mouse Ly-49A/D (Ly49A/D)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio12A8 antibody reacts with Ly-49A and Ly-49D. The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Ly-49D is expressed on subsets of natural killer cells in C57BL/6, C3H/He, and SJL mice, but not DBA/2, AKR, CBA/J, or BALB/c mice. The Ly-49D antigen has not been detected on NK-1.1+ T cells. The Ly-49D receptor has been implicated in mediation of NK cell cytolysis. Ly-49D is an activating receptor while Ly-49A is a inhibitory receptor.

eBio12A8 does not react with BALB/c, DBA/2, AKR, or CBA/J Ly-D49D.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio12A8 (12A8) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested

This eBio12A8 (12A8) antibody has been tested by flow cytometric analysis of cultured mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Direct conjugates are recommended for flow cytometric analysis.

References

Mason LH, Anderson SK, Yokoyama WM, Smith HR, Winkler-Pickett R, Ortaldo JR. The Ly-49D receptor activates murine natural killer cells. J Exp Med. 1996 Dec 1;184(6):2119-28. (PubMed)

Ortaldo, J.R., et al. 1998. The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. J. Immunol. 160: 1158 - 1165.

Takei, F., et al. 1997. The Ly-49 family: genes, proteins and recognition of class I MHC. Immunol. Rev. 155: 67 – 77.

Ortaldo, J.R., et al. 1999. Ly-49 receptor expression and functional analysis in multiple mouse strains. J. Leukoc. Biol. 66: 512 - 520.

Nakamura MC, Hayashi S, Niemi EC, Ryan JC, Seaman WE. Activating Ly-49D and inhibitory Ly-49A natural killer cell receptors demonstrate distinct requirements for interaction with H2-D(d). J Exp Med. 2000 Aug 7;192(3):447-54. (PubMed)

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321  Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)