Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
The hNanog.1 antibody was generated against and reacts with human Nanog, a homeobox transcription factor. In addition to the known Oct4 and Stat3 transcriptional operating systems, Nanog plays a crucial role in the second embryonic cell fate specification following formation of the blastocyst. Nanog was identified as a gene specifically expressed in mouse ES cells and preimplantation embryos and is capable of maintaining ES cell self-renewal independently of LIF/Stat3. Potential cross-reactivity of this antibody to other proteins has not been determined.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This hNanog.1 antibody has been reported for use in immunoblotting (WB), and immunohistology staining of paraffin embedded tissue sections.
Applications Tested
The hNanog.1 antibody has been tested by immunoblotting (WB) of Ntera cell lysates (1-2ug/ml).
References
Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S. 2003. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 113(5):631-42.
Cavaleri F, Scholer HR. 2003. Nanog: a new recruit to the embryonic stem cell orchestra. Cell. 113(5):551-2
Hoei-Hansen CE, Kraggerud SM, et al. 2007. Ovarian dysgerminomas are characterised by frequent KIT mutations and abundant expression of pluripotency markers. Mol Cancer 2;6:12. (hNanog.1, IHC paraffin, PubMed)
