Product Information

Contents: Affinity Purified anti-human Nanog
Catalog Number: 14-5768
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: hNanog.2
Isotype: Mouse IgG1, κ

NTERA cell lysate was loaded at 1x10⁵ cells/lane, probed with 1 µg/mL purified hNANOG.2 and revealed with HRP anti-mouse IgG.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5768	Affinity Purified Mouse anti-human Nanog monoclonal	N/A	N/A	WB

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Description

The hNanog.2 antibody was generated against and reacts with human Nanog, a homeobox transcription factor. In addition to the known Oct4 and Stat3 transcriptional operating systems, Nanog plays a crucial role in the second embryonic cell fate specification following formation of the blastocyst. Nanog was identified as a gene specifically expressed in mouse ES cells and preimplantation embryos and is capable of maintaining ES cell self-renewal independently of LIF/Stat3. Potential cross-reactivity of this antibody to other proteins has not been determined.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This hNanog.2 antibody has been reported for use in immunoblotting (WB).

Applications Tested

This hNanog.2 antibody has been tested by immunoblotting of NTERA cell lysates.

References

Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S. 2003. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 113(5):631-42.
Cavaleri F, Scholer HR. 2003. Nanog: a new recruit to the embryonic stem cell orchestra. Cell. 113(5):551-2

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