Product Information

Contents: Affinity Purified anti-mouse Nanog
Catalog Number: 14-5761
Sizes: 25 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioMLC-51
Isotype: Rat IgG2a

Lysates from the F9 cell line (left), the NIH3T3 cell line (middle), and Balb/C spleen (right) were probed with 1 μg/ml of purified eBioMLC51 and revealed with HRP anti-rat IgG.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5761	Affinity Purified anti-mouse Nanog	N/A	N/A	WB
51-5761	Alexa Fluor® 647 anti-mouse Nanog	633	668	IC Flow
53-5761	Alexa Fluor® 488 anti-mouse Nanog	488	519	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioMLC51 monoclonal antibody recognizes mouse Nanog. Nanog is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of Nanog in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Nanog expression often overlaps, but is not identical to, that of Oct4. Nanog is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells.

Nanog acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to Nanog, whereas the NK2 DNA binding domain is well conserved.

Immunoblotting using eBioMLC51 reveals a band at ~ 45 kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen.

Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Cat. 00-5523, for intracellular staining of mouse Nanog.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioMLC-51 antibody has been reported for use in immunoblotting (WB).

Applications Tested

This eBioMLC-51 antibody has been tested by immunoblotting the F9 cell line. This purified antibody can be used at 2 μg/ml, however should be titrated for optimal performance.

References

Mitsui, K., Tokuzawa, Y., Itoh, H., Segawa, K., Murakami, M., Takahashi, K., Maruyama, M., Maeda, M., Yamanaka, S. 2003. The Homeoprotein Nanog is Required for Maintenance of Pluripotency in Mouse Epiblast and ES Cells. Cell 113:631-642

Yanmei Chen, Zhongwei Du, Zhen Yao. 2006. Roles of the Nanog Protein in Murine F9 Embryonal Carcinoma Cells and Their Endoderm-Differentiated Counterparts. Cell Research 16:641-650.

Guangjin Pan and Duanqing Pei. 2005. The Stem Cell Pluripotency Factor NANOG Activates Transcription with Two Unuasually Potent Subdomains at Its C Terminus. The Journal of Biological Chemistry Vol.280, No.2, 1401-1407.

Related Products

Cat. 14-5768 Affinity Purified Mouse anti-human Nanog monoclonal (clone hNanog.2)
Cat. 14-5769 Affinity Purified Mouse anti-human Nanog monoclonal (clone hNanog.1)