Product Information

Contents: Affinity Purified anti-mouse RAE-1d (RAE-1-delta, RAE-1-d, RAE1-d)
Catalog Number: 14-5756
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: RD-41
Isotype: Mouse IgG1

Staining of dendritic cell line with 0.125 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.125 μg of Purified anti-mouse RAE-1d (RD-41) (colored histogram) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5756	Affinity Purified anti-mouse RAE-1d (RAE-1-delta, RAE-1-d, RAE1-d)	N/A	N/A	ELISA FA FC IH/F IHC
12-5756	Phycoerythrin (PE) anti-mouse RAE-1d (RAE-1-delta, RAE-1-d, RAE1-d)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RD-41 monoclonal antibody reacts with the murine Rae1δ. Rae-1δ is one of several known murine NKG2D ligands that include RAE-1 molecules (α, β, ε, γ and δ), H60 and MULT-1. Expression of NKG2D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2D ligands which in turn activates NK cells tumoricidal activity through NKG2D. Until now, the expression of NKG2D ligands has been mainly studied with NKG2D tetramers which recognizes all NKG2D ligands. The RD-41 antibody has been reported to block tetramer staining.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RD-41 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections and ELISA.

Applications Tested

This RD-41 antibody has been tested by flow cytometric analysis of dendritic cell line. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Diefenbach A, Jamieson AM, Liu SD, Shastri N, Raulet DH. Ligands for the murine NKG2D receptor: expression by tumor cells and activation of NK cells and macrophages. Nat Immunol. 2000 Aug;1(2):119-26.

Cerwenka A, Bakker AB, McClanahan T, Wagner J, Wu J, Phillips JH, Lanier LL. Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice. Immunity. 2000 Jun;12(6):721-7.

O'Callaghan CA, Cerwenka A, Willcox BE, Lanier LL, Bjorkman PJ. Molecular competition for NKG2D: H60 and RAE1 compete unequally for NKG2D with dominance of H60. Immunity. 2001 Aug;15(2):201-11.

Diefenbach A, Jensen ER, Jamieson AM, Raulet DH. Rae1 and H60 ligands of the NKG2D receptor stimulate tumour immunity. Nature. 2001 Sep 13;413(6852):165-71.

Carayannopoulos LN, Naidenko OV, Kinder J, Ho EL, Fremont DH, Yokoyama WM. Ligands for murine NKG2D display heterogeneous binding behavior. Eur J Immunol. 2002 Mar;32(3):597-605.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 12-4012  Phycoerythrin (PE) Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (clone polyclonal)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control