Product Information

Contents: Affinity Purified anti-mouse JAML (Junctional Adhesion Molecule-Like protein)
Catalog Number: 14-5666
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio4E10 (4E10)
Isotype: Armenian Hamster IgG

Staining of mouse JAML-transfected CHO cells with 0.5 μg of Purified Armenian Hamster IgG Iso Cntrl (cat. 14-4888) (open histogram) or 0.5 μg of Purified anti-mouse JAML (4E10) (colored histogram) followed by FITC Anti-Armenian Hamster IgG (cat. 11-4111). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5666	Affinity Purified anti-mouse JAML (Junctional Adhesion Molecule-Like)	N/A	N/A	FC IP
51-5666	Alexa Fluor® 647 anti-mouse JAML (Junctional Adhesion Molecule-Like)	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio4E10 monoclonal antibody reacts with mouse JAML (junctional adhesion molecule-like protein), also known as AMICA. JAML is a glycosylated membrane protein possessing two Ig-like domains in its extracellular domain. JAML is expressed on polymorphonuclear leukocytes (PMN), and is involved in the transmigration of such cells through epithelial tight junctions via interactions between JAML and epithelial coxsackie and adenovirus receptor (CAR). JAML is also expressed on lymphocyte subsets, such as γδ T cell lines, and can provide co-stimulatory signals for cell activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio4E10 antibody has been reported for use in flow cytometric analysis and immunoprecipitation.

Applications Tested

This eBio4E10 (4E10) antibody has been tested by flow cytometric analysis of mouse lysed whole blood. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Zen K, Liu Y, McCall IC, Wu T, Lee W, Babbin BA, Nusrat A, Parkos CA. Neutrophil migration across tight junctions is mediated by adhesive interactions between epithelial coxsackie and adenovirus receptor and a junctional adhesion molecule-like protein on neutrophils. Mol Biol Cell. 2005 Jun;16(6):2694-703. (PubMed)

Moog-Lutz C, Cave-Riant F, Guibal FC, Breau MA, Di Gioia Y, Couraud PO, Cayre YE, Bourdoulous S, Lutz PG. JAML, a novel protein with characteristics of a junctional adhesion molecule, is induced during differentiation of myeloid leukemia cells. Blood. 2003 Nov 1;102(9):3371-8. (PubMed)

Related Products

Cat. 11-4111  FITC Anti-Armenian Hamster IgG
Cat. 13-4113  Biotin Anti-Armenian Hamster IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4888  Affinity Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm)