Immunoblotting of reduced lysates from C2C12 cells using 5 µg/mL of Anti-Myogenin Purified (left). Bands were visualized using of Anti-Mouse IgG HRP. Immunocytochemistry of fixed and permeabilized C2C12 cell line using 1 µg/ml of Anti-Myogenin Purified antibody followed by Anti-Mouse IgG FITC (cat. 11-4011) (right). Phase/fluorescence overlay (center).
Contents: Anti-Myogenin Purified Catalog Number: 14-5643 Concentration: 0.5 mg/ml Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Clone: F5D Host/Isotype: Mouse IgG1
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Size
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14-5643-80
25 ug
14-5643-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
This F5D monoclonal antibody reacts with human, mouse, dog, and rat myogenin, a 34-kDa transcription factor. Expressed in skeletal and heart muscle, myogenin is a member of the MyoD family of basic-helix-loop-helix proteins, which also includes MyoD, Myf5, and MRF4. This transcription factor interacts with other helix-loop-helix proteins, which may or may not be muscle-specific. Myogenin plays a significant role in myogenic differentiation, even directing nonmuscle cells to the myogenic lineage. Transforming growth factor-beta (TGFb) and bone morphogenetic protein-2 (BMP2) inhibit myogenin transcriptional activity. Predominantly residing within the nucleus, the subcellular localization of myogenin has been shown to be dependent on differentiation status and cell density. For instance, trafficking of myogenin between the nucleus and cytoplasm has been reported during skeletal muscle differentiation to mediate transcription control.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This F5D antibody has been reported for use in immunoblotting (WB) and immunocytochemistry.
Applications Tested
This F5D antibody has been tested by western blot and immunocytochemistry of the C2C12 cell line. This can be used at less than or equal to 1-5 ug/mL for both applications. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ferri P, Barbieri E, Burattini S, Guescini M, D'Emilio A, Biagiotti L, Del Grande P, De Luca A, Stocchi V, Falcieri E. Expression and subcellular localization of myogenic regulatory factors during the differentiation of skeletal muscle C2C12 myoblasts. J. Cell Biochem. 2009 Oct 14.
Li D, Niu Z, Yu W, Qian Y, Wang Q, Li Q, Yi Z, Luo J, Wu X, Wang Y, Schwartz RJ, Liu M. SMYD1, the myogenic activator, is a direct target of serum response factor and myogenin. Nucleic Acids Res. 2009 Sep 25.
Lindon C, Albagli O, Pinset C, Montarras D. Cell density-dependent induction of endogenous myogenin (myf4) gene expression by Myf5. Dev Biol. 2001 Dec 15;240(2):574-84.
Wright WE, Dac-Korytko I, Farmer K. Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interactions. Dev Genet. 1996;19(2):131-8. (F5D, WB)
Chakraborty T, Martin JF, Olson EN. Analysis of the oligomerization of myogenin and E2A products in vivo using a two-hybrid assay system. J Biol Chem. 1992 Sep 5;267(25):17498-501.
Martin JF, Li L, Olson EN. Repression of myogenin function by TGF-beta 1 is targeted at the basic helix-loop-helix motif and is independent of E2A products. J Biol Chem. 1992 Jun 5;267(16):10956-60.
