Staining of human TSLPR-transfected Ba/F3 cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human TSLPR (1D8) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Affinity Purified anti-human TSLP Receptor (TSLPR, thymic stromal-derived lymphopoietin receptor) Catalog Number: 14-5498 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Clone: eBio1D8 (1D8) Host/Isotype: Mouse IgG1, λ Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles.
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Cat. No.
Size
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14-5498-80
25 ug
14-5498-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The eBio1D8 monoclonal antibody reacts with human thymic stromal-derived lymphopoietin receptor (TSLPR). TSLPR is an approximately 50 kDa protein with significant similarity to the common γ-chain (CD132). TSLPR complexes with IL-7Rα (CD127) to form the high affinity receptor that binds thymic stromal-derived lymphopoietin (TSLP). Human TSLPR is expressed by monocytes and CD11c+ dendritic cells, and TSLP binding induces the expression of the TH2 cell-attracting chemokines CCL17 and CCL22. Furthermore, the TSLPR-induced activation of dendritic cells indirectly results in the increased secretion of TH2 cytokines IL-4, -5 and -13, which may be necessary for the regulation of CD4+ T cell homeostasis. In mice, deficiency of TSLPR has no effect on lymphocyte numbers, whereas double deficiency of TSLPR and common γ-chain results in fewer lymphocytes than seen in mice deficient in the common γ-chain alone. The eBio1D8 monoclonal antibody is able to cross-block binding of another anti-human TSLPR monoclonal antibody, eBio1A6.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio1D8 (1D8) antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB).
Applications Tested
This eBio1D8 (1D8) antibody has been tested by flow cytometric analysis of human TSLPR-transfected Ba/F3 cells. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Reche PA, Soumelis V, Gorman DM, Clifford T, Liu Mr, Travis M, Zurawski SM, Johnston J, Liu YJ, Spits H, de Waal Malefyt R, Kastelein RA, Bazan JF.
Human thymic stromal lymphopoietin preferentially stimulates myeloid cells.
J Immunol. 2001 Jul 1;167(1):336-43.
Soumelis V, Reche PA, Kanzler H, Yuan W, Edward G, Homey B, Gilliet M, Ho S, Antonenko S, Lauerma A, Smith K, Gorman D, Zurawski S, Abrams J, Menon S, McClanahan T, de Waal-Malefyt Rd R, Bazan F, Kastelein RA, Liu YJ.
Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP.
Nat Immunol. 2002 Jul;3(7):673-80.
