Product Information

Contents: Affinity Purified anti-human GITR / AITR Ligand
Catalog Number: 14-5475
Sizes: 25 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioAITR-L
Isotype: Mouse IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5475	Affinity Purified anti-human GITR / AITR Ligand (GITRL, GITR-L, AITRL, AITR-L)	N/A	N/A	ELISA FC
12-5475	Phycoerythrin (PE) anti-human GITR / AITR Ligand (GITRL, GITR-L, AITRL, AITR-L)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioAITR-L monoclonal antibody reacts with human Activation-Induced TNFR family member ligand (AITRL), also known as TL6. AITRL is a type II transmembrane protein of the TNF superfamily. AITRL is expressed by endothelial cells and peripheral blood monocytes. Interaction of AITR with AITRL is important for cross-talk between T lymphocytes and endothelial cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioAITR-L antibody has been reported for use in flow cytometric analysis, and ELISA.

Applications Tested

This eBioAITR-L antibody has been tested by flow cytometric analysis of human umbilical vein endothelial cells (HUVEC). This can be used at less than or equal to 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kwon B, Yu KY, Ni J, Yu GL, Jang IK, Kim YJ, Xing L, Liu D, Wang SX, Kwon BS. 1999. J Biol Chem. 274(10):6056-61. Identification of a novel activation-inducible protein of the tumor necrosis factor receptor superfamily and its ligand.

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