mouse/rat 3G11 (sialoganglioside antigen) antibody: purified antibody

Contents: Affinity Purified anti-mouse/rat 3G11 sialoganglioside antigen
Catalog Number: 14-5443
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio3G11 (SM3G11, 3G11)
Isotype: Mouse IgM, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-5443-81	50 ug	$65
14-5443-82	100 ug	$90
14-5443-85	500 ug	$235

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c thymocytes with 0.5 μg of Purified Mouse IgM Iso Cntrl (cat. 14-4752) (open histogram) or 0.5 μg of Purified anti-mouse 3G11 (SM3G11) (colored histogram) followed by PE anti-mouse IgM (II/41) (cat. 12-5790). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5443	Affinity Purified anti-mouse/rat 3G11 sialoganglioside antigen	N/A	N/A	FC
51-5443	Alexa Fluor® 647 anti-mouse/rat 3G11 sialoganglioside antigen	633	668	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio3G11 monoclonal antibody reacts with the mouse 3G11antigen. The 3G11 antibody was isolated as an autoantibody against the 3G11 sialylated carbohydrate antigen, which was found to be expressed by thymoyctes and peripheral T cells, but not by B cells, myeloid cells or erythrocytes. 3G11 is expressed at higher levels on CD4+ T cells than on CD8+ T cells, however expression on both of these subsets decreases with advancing age. In thymus, 3G11 is expressed on approximately 20% of total thymocytes. 3G11 has also been used as a marker to distinguish naïve and memory T cells, as 3G11 is irreversibly down-regulated upon activation of CD4+ T cells. The 3G11 antibody is able to block binding of antibodies to CD3ε, suggesting close proximity of these two proteins.

Recently, it has been demonstrated that loss of 3G11 expression in experimental autoimmune encephalomyelitis (EAE) is associated with an anergic, regulatory T phenotype. Purified 3G11- T cells were able to suppress proliferation and cytokine production of 3G11+ T cells, and part of this effect was due to the secretion of IL-10.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio3G11 (SM3G11, 3G11) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio3G11 (SM3G11, 3G11) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hayakawa K, Hardy RR. Murine CD4+ T cell subsets defined. J Exp Med. 1988 Nov 1;168(5):1825-38. (3G11, FC, PubMed)

Zhang GX, Yu S, Calida D, Zhao Z, Gran B, Kamoun M, Rostami A. Loss of the surface antigen 3G11 characterizes a distinct population of anergic/regulatory T cells in experimental autoimmune encephalomyelitis. J Immunol. 2006 Mar 15;176(6):3366-73. (3G11, FC, PubMed)

Ernst DN, Hobbs MV, Torbett BE, Glasebrook AL, Rehse MA, Bottomly K, Hayakawa K, Hardy RR, Weigle WO. Differences in the expression profiles of CD45RB, Pgp-1, and 3G11 membrane antigens and in the patterns of lymphokine secretion by splenic CD4+ T cells from young and aged mice. J Immunol. 1990 Sep 1;145(5):1295-302. (3G11, FC, PubMed)

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