Product Information

Contents: Affinity Purified anti-mouse Podoplanin (T1a, gp38, stromal cell marker)
Catalog Number: 14-5381
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio8.1.1 (8.1.1)
Isotype: Golden Syrian Hamster IgG

Staining of TE-71 cells with 0.25 μg of Purified Golden Syrian Hamster IgG Iso Cntrl (cat. 14-4914) (open histogram) or 0.25 μg of Purified anti-mouse Podoplanin (8.1.1) (colored histogram) followed by Biotin Anti-Syrian Hamster IgG (cat. 13-4213)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5381	Affinity Purified anti-mouse Podoplanin (T1a, gp38, stromal cell marker)	N/A	N/A	FC IHC IP WB
53-5381	Alexa Fluor® 488 anti-mouse Podoplanin (T1a, gp38, stromal cell marker)	488	519	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein, named for its expression in kidney glomerular epithelial cells (podocytes). In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum. It was also shown to be a potent marker for lymphatic endothelium. Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of TE-71 cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Farr A, Nelson A, Hosier S. Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus. J Histochem Cytochem. 1992 May;40(5):651-64. (8.1.1, mAb development, PubMed)

Farr AG, Berry ML, Kim A, Nelson AJ, Welch MP, Aruffo A. Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues. J Exp Med. 1992 Nov 1;176(5):1477-82. (8.1.1, IHC, PubMed)

Mahtab EA, Wijffels MC, Van Den Akker NM, Hahurij ND, Lie-Venema H, Wisse LJ, Deruiter MC, Uhrin P, Zaujec J, Binder BR, Schalij MJ, Poelmann RE, Gittenberger-De Groot AC. Cardiac malformations and myocardial abnormalities in podoplanin knockout mouse embryos: Correlation with abnormal epicardial development. Dev Dyn. 2008 Mar;237(3):847-57.

Related Products

Cat. 11-4211  FITC Anti-Syrian Hamster IgG (clone Polyclonal)
Cat. 13-4213  Biotin Anti-Syrian Hamster IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4914  Affinity Purified Golden Syrian Hamster IgG Isotype Control (clone n/a)