Product Information

Contents: Affinity Purified anti-mouse Galectin-3 / Mac-2
Catalog Number: 14-5301
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioM3/38 (M3/38)
Isotype: Rat IgG2a, κ

Staining of mouse PECs with 0.25 µg of rat IgG2a (cat. 14-4321) (open) or 0.25 μg anti-galectin-3 (closed) followed by Sav-APC (cat. 17-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5301	Affinity Purified anti-mouse Galectin-3 / Mac-2	N/A	N/A	ELISA FC IH/F IHC(Paraffin) IP WB
13-5301	Biotin anti-mouse Galectin-3 / Mac-2	N/A	N/A	FC
12-5301	Phycoerythrin (PE) anti-mouse Galectin-3 / Mac-2	488	575	FC
51-5301	Alexa Fluor® 647 anti-mouse Galectin-3 / Mac-2	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.

This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen tissue sections, immunohistology staining of paraffin embedded tissue sections, and ELISA.

Applications Tested

This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse PECs (thioglycollated elicied peritioneal cells). This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ho MK, Springer TA. 1982. MAC-2, A Novel 32000 Mr Mouse Macrophage Subpopulation-Specific Antigen Defined by Monoclonal Antibodies. J. Immunol. 128(3):1221-1228.

Joo G-H, Goedegebuure PS, Sadanaga N, Nagoshi M, von Bernstorff W, Eberlein TJ. Expression and function of galectin-3, a b-galactoside-binding protein in activated T lymphocytes J. Leukoc. Biol. 69: 555–564; 2001.

Oliveira FL, Fraza P, Chammas R, Hsu DK, Liu FT, Borojevic R, Takiya CM, El-Cheikh MC, Kinetics of mobilization and differentiation of lymphohematopoietic cells during experimental murine schistosomiasis in galectin-3-/- mice J. Leukoc. Biol. 82: ePub; 2007

Springer TA, 1981. Monoclonal Antibody Analysis of Complex Biological Systems. J. Biol Chem. 256(8):3833-3839.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321  Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)