Product Information

Contents: Affinity Purified Mouse IgG2b Isotype Control
Catalog Number: 14-4732
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Isotype: Mouse IgG2b, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-4732	Affinity Purified Mouse IgG2b Isotype Control	N/A	N/A	FC
16-4732	Functional Grade* Purified Mouse IgG2b Isotype Control	N/A	N/A	FC
13-4732	Biotin Mouse IgG2b Isotype Control	N/A	N/A	FC
11-4732	FITC Mouse IgG2b Isotype Control	488	518	FC
12-4732	PE Mouse IgG2b Isotype Control	488	575	FC
15-4732	Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG2b Isotype Control	488	670	FC
17-4732	Allophycocyanin (APC) Mouse IgG2b Isotype Control	633	660	FC
25-4732	Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG2b Isotype Control	488	760	FC
35-4732	Phycoerythrin-Cy5.5 (PE-Cy5.5) Mouse IgG2b Isotype Control	488	690	FC
51-4732	Alexa Fluor® 647 Mouse IgG2b Isotype Control	633	668	FC
53-4732	Alexa Fluor® 488 Mouse IgG2b Isotype Control	488	519	FC
56-4732	Alexa Fluor® 700 Mouse IgG2b Isotype Control (APC-Cy5.5 Equivalent)	633	723	FC
57-4732	Pacific Blue® Mouse IgG2b Isotype Control	402	421	FC
27-4732	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG2b Isotype Control (APC-Cy7 Equivalent)	633	779	FC
45-4732	PerCP-Cy5.5 Mouse IgG2b Isotype Control	488	690	FC
93-4732	eFluor^TM 605^NC Mouse IgG2b Isotype Control	350-500	605	FC
94-4732	eFluor^TM 625^NC Mouse IgG2b Isotype Control	350-500	625	FC
95-4732	eFluor^TM 650^NC Mouse IgG2b Isotype Control	350-500	650	FC
47-4732	Coming Soon! - Allophycocyanin-eFluor^TM 780 Mouse IgG2b Isotype Control (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-4732	eFluor^TM 450 Mouse IgG2b Isotype Control (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal mouse IgG2b, κ is useful as an isotype control immunoglobulin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This mouse IgG2b isotype control has been reported for use in surface and intracellular flow cytometric analysis.

Applications Tested

This mouse IgG2b isotype control has been tested by flow cytometric analysis of human peripheral blood leukocytes and can be used at less than or equal to 1 µg per 100 µl blood. It is recommended that the antibody be carefully titrated for optimal performance in other applications.