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Affinity Purified Mouse IgG2a Isotype Control

 
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Contents: Affinity Purified Mouse IgG2a Isotype Control
Catalog Number: 14-4724
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Isotype: Mouse IgG2a, κ

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14-4724-81 50 ug
14-4724-82 100 ug
14-4724-85 500 ug


   

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
14-4724 Affinity Purified Mouse IgG2a, K Isotype Control N/A N/A FC 
16-4724 Functional Grade* Purified Mouse IgG2a, K Isotype Control N/A N/A FC 
13-4724 Biotin Mouse IgG2a, K Isotype Control N/A N/A FC 
11-4724 Fluorescein isothiocyanate (FITC) Mouse IgG2a, K Isotype Control 488 518 FC 
12-4724 Phycoerythrin (PE) Mouse IgG2a, K Isotype Control 488 575 FC 
15-4724 Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG2a, K Isotype Control 488 670 FC 
17-4724 Allophycocyanin (APC) Mouse IgG2a, K Isotype Control 633 660 FC 
25-4724 Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG2a, K Isotype Control 488 760 FC 
51-4724 Alexa Fluor® 647 Mouse IgG2a, K Isotype Control 633 668 FC 
53-4724 Alexa Fluor® 488 Mouse IgG2a, K Isotype Control 488 519 FC 
56-4724 Alexa Fluor® 700 Mouse IgG2a, K Isotype Control (APC-Cy5.5 Equivalent) 633 723 FC 
57-4724 Pacific Blue® Mouse IgG2a, K Isotype Control 402 421 FC 
27-4724 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG2a, K Isotype Control (APC-Cy7 Equivalent) 633 779 FC 
45-4724 PerCP-Cy5.5 Mouse IgG2a, K Isotype Control 488 690 FC 
93-4724 eFluorTM 605NC Mouse IgG2a, Isotype Control 350-500 605 FC 
94-4724 eFluorTM 625NC Mouse IgG2a Isotype Control 350-500 625 FC 
95-4724 eFluorTM 650NC Mouse IgG2a, K Isotype Control 350-500 650 FC 
47-4724 Allophycocyanin-eFluorTM 780 Mouse IgG2a, K Isotype Control (APC-Alexa Fluor® 750 replacement) 633 780 FC 
48-4724 eFluorTM 450 Mouse IgG2a, K Isotype Control (Pacific Blue® replacement) 405 450 FC 
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description


This is a monoclonal mouse IgG2a, κ antibody. It is used as an isotype control for mouse IgG2a antibodies.


Applications Reported


For research use only, not for diagnostic or therapeutic use. This mouse IgG2a isotype control has been reported for use in surface and intracellular flow cytometric analysis.


Applications Tested


This mouse IgG2a isotype control has been tested by flow cytometric analysis of human peripheral blood leukocytes and can be used at less than or equal to 1 µg per 100 µl blood. It is recommended that the antibody be carefully titrated for optimal performance in assay of interest.


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