Contents: Affinity Purified Mouse IgG1 Isotype Control
Catalog Number: 14-4714
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Isotype: Mouse IgG1, κ
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-4714 |
Affinity Purified Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
IC Flow
IP
WB
|
| 16-4714 |
Functional Grade* Purified Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
|
| 13-4714 |
Biotin Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
|
| 11-4714 |
Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control |
488 |
518 |
FC
|
| 12-4714 |
Phycoerythrin (PE) Mouse IgG1, K Isotype Control |
488 |
575 |
FC
|
| 15-4714 |
Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control |
488 |
670 |
FC
|
| 17-4714 |
Allophycocyanin (APC) Mouse IgG1, K Isotype Control |
633 |
660 |
FC
|
| 25-4714 |
Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control |
488 |
760 |
FC
|
| 35-4714 |
Phycoerythrin-Cy5.5 (PE-Cy5.5) Mouse IgG1, K Isotype Control |
488 |
690 |
FC
|
| 51-4714 |
Alexa Fluor® 647 Mouse IgG1, K Isotype Control |
633 |
668 |
FC
|
| 53-4714 |
Alexa Fluor® 488 Mouse IgG1, K Isotype Control |
488 |
519 |
FC
|
| 56-4714 |
Alexa Fluor® 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-4714 |
Pacific Blue® Mouse IgG1, K Isotype Control |
402 |
421 |
FC
|
| 27-4714 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG1, K Isotype Control (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 45-4714 |
PerCP-Cy5.5 Mouse IgG1, K Isotype Control |
488 |
690 |
FC
|
| 93-4714 |
eFluorTM 605NC Mouse IgG1, Isotype Control |
350-500 |
605 |
FC
|
| 94-4714 |
eFluorTM 625NC Mouse IgG1 Isotype Control |
350-500 |
625 |
FC
|
| 95-4714 |
eFluorTM 650NC Mouse IgG1, K Isotype Control |
350-500 |
650 |
FC
|
| 47-4714 |
Allophycocyanin-eFluorTM 780 Mouse IgG1, K Isotype Control (APC-Alexa Fluor® 750 replacement) |
633 |
780 |
FC
|
| 48-4714 |
eFluorTM 450 Mouse IgG1, K Isotype Control (Pacific Blue® replacement) |
405 |
450 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The monoclonal mouse IgG1, κ, immunoglobulin is useful as an isotype control.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This mouse IgG1 isotype control has been reported for use in surface and intracellular flow cytometric analysis, immunohistochemcial staining, immunoprecipitation and immunoblotting (WB).
Applications Tested
This mouse IgG1 isotype control has been tested by flow cytometric analysis of human peripheral leukocytes and can be used at less than or equal to 1 µg per 100 µl blood. It is recommended that the antibody be carefully titrated for optimal performance in other applications.
Related Products
Cat. 11-4011 FITC Anti-Mouse IgGCat. 13-4013 Biotin Anti-Mouse IgG (clone Polyclonal)Cat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate)Cat. 12-4317 Streptavidin-PE (Phycoerythrin)Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC)Cat. 18-8877 See Catalog # 18-8817 for an alternative product: Mouse TrueBlot™: Horseradish Peroxidase (HRP) anti-mouse IgG
