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Affinity Purified Mouse IgG1 Isotype Control

 
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Contents: Affinity Purified Mouse IgG1 Isotype Control
Catalog Number: 14-4714
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Isotype: Mouse IgG1, κ

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14-4714-81 50 ug
14-4714-82 100 ug
14-4714-85 500 ug


   

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
14-4714 Affinity Purified Mouse IgG1, K Isotype Control N/A N/A FC  IC Flow  IP  WB 
16-4714 Functional Grade* Purified Mouse IgG1, K Isotype Control N/A N/A FC 
13-4714 Biotin Mouse IgG1, K Isotype Control N/A N/A FC 
11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control 488 518 FC 
12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control 488 575 FC 
15-4714 Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control 488 670 FC 
17-4714 Allophycocyanin (APC) Mouse IgG1, K Isotype Control 633 660 FC 
25-4714 Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control 488 760 FC 
35-4714 Phycoerythrin-Cy5.5 (PE-Cy5.5) Mouse IgG1, K Isotype Control 488 690 FC 
51-4714 Alexa Fluor® 647 Mouse IgG1, K Isotype Control 633 668 FC 
53-4714 Alexa Fluor® 488 Mouse IgG1, K Isotype Control 488 519 FC 
56-4714 Alexa Fluor® 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent) 633 723 FC 
57-4714 Pacific Blue® Mouse IgG1, K Isotype Control 402 421 FC 
27-4714 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG1, K Isotype Control (APC-Cy7 Equivalent) 633 779 FC 
45-4714 PerCP-Cy5.5 Mouse IgG1, K Isotype Control 488 690 FC 
93-4714 eFluorTM 605NC Mouse IgG1, Isotype Control 350-500 605 FC 
94-4714 eFluorTM 625NC Mouse IgG1 Isotype Control 350-500 625 FC 
95-4714 eFluorTM 650NC Mouse IgG1, K Isotype Control 350-500 650 FC 
47-4714 Allophycocyanin-eFluorTM 780 Mouse IgG1, K Isotype Control (APC-Alexa Fluor® 750 replacement) 633 780 FC 
48-4714 eFluorTM 450 Mouse IgG1, K Isotype Control (Pacific Blue® replacement) 405 450 FC 
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description


The monoclonal mouse IgG1, κ, immunoglobulin is useful as an isotype control.


Applications Reported


For research use only, not for diagnostic or therapeutic use. This mouse IgG1 isotype control has been reported for use in surface and intracellular flow cytometric analysis, immunohistochemcial staining, immunoprecipitation and immunoblotting (WB).


Applications Tested


This mouse IgG1 isotype control has been tested by flow cytometric analysis of human peripheral leukocytes and can be used at less than or equal to 1 µg per 100 µl blood. It is recommended that the antibody be carefully titrated for optimal performance in other applications.


Related Products


Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 18-8877  See Catalog # 18-8817 for an alternative product: Mouse TrueBlot™: Horseradish Peroxidase (HRP) anti-mouse IgG


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