Product Information

Contents: Purified anti-Phycoerythrin (anti-PE)
Catalog Number: 14-4120
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioPE-DLF
Isotype: Mouse IgG1, κ

Staining of normal human PBMCs with PE anti-mo/hu CD44 (IM7) (cat. 12-0441), followed by 0.125 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (left) or 0.125 μg of Purified anti-PE (right), followed by detection with FITC Anti-Mouse IgG (cat. 11-4011). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-4120	Purified anti-Phycoerythrin (anti-PE)	N/A	N/A	FC
13-4120	Biotin anti-Phycoerythrin (anti-PE)	N/A	N/A	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioPE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using eBioPE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PE-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The eBioPE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioPE-DLF antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioPE-DLF antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Guy K, Crichton DN, Ross JA. Indirect immunofluorescence labelling with complexes of phycoerythrin and monoclonal anti-phycoerythrin antibodies (PEAPE complexes). J Immunol Methods. 1988 Sep 13;112(2):261-5.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 12-4012  Phycoerythrin (PE) Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (clone polyclonal)
Cat. 17-4012  Allophycocyanin (APC) Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (clone polyclonal)
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4732  Affinity Purified Mouse IgG2b Isotype Control