Product Information

Contents: Affinity Purified anti-CD324 (E-cadherin, Ecad, epithelial cadherin)
Catalog Number: 14-3249
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: DECMA-1
Isotype: Rat IgG1

Immunoblot analysis of reduced MDCK lysates with 2μg/ml purified anti-E-cadherin (DECMA-1) antibody and revealed with HRP anti-rat IgG. Lane 1: EDTA treated cells and Lane 2: Trypsin treated cells to remove from tissue culture dish.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-3249	Affinity Purified anti-CD324 (E-cadherin,epithelial cadherin)	N/A	N/A	ELISA FA FC IHC IP WB
51-3249	Alexa Fluor® 647 anti- CD324 (E-cadherin, epithelial cadherin )	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton. Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintenance of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers.

E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided. The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This DECMA-1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunocytochemistry, and immunohistochemical staining. (Please use Functional Grade purified DECMA-1, cat. 16-3249, in functional assays.)

Applications Tested

This DECMA-1 antibody has been tested by western blot analysis of EDTA treated MDCK cell line. This can be used at less than or equal to 2μg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Spencer HL, Eastham AM, Merry CL, Southgate TD, Perez-Campo F, Soncin F, Ritson S, Kemler R, Stern PL, Ward CM. E-cadherin inhibits cell surface localization of the pro-migratory 5T4 oncofetal antigen in mouse embryonic stem cells. Mol Biol Cell. 2007 Aug;18(8):2838-51. (DECMA-1, FC, WB, FA, (in mouse))

Nakagawa M, Fukata M, Yamaga M, Itoh N, Kaibuchi K. Recruitment and activation of Rac1 by the formation of E-cadherin-mediated cell-cell adhesion sites. J Cell Sci. 2001 May;114(Pt 10):1829-38. (DECMA-1, FA in canine cells, PubMed)

Lee MG, Sharrow SO, Farr AG, Singer A, Udey MC. Expression of the homotypic adhesion molecule E-cadherin by immature murine thymocytes and thymic epithelial cells. J Immunol. 1994 Jun 15;152(12):5653-9. (DECMA-1, FC in mouse cells)

Tang A, Eller MS, Hara M, Yaar M, Hirohashi S, Gilchrest BA. E-cadherin is the major mediator of human melanocyte adhesion to keratinocytes in vitro. J Cell Sci. 1994 Apr;107 ( Pt 4):983-92. (DECMA-1, FC on human cells)

Vestweber D, Kemler R. Identification of a putative cell adhesion domain of uvomorulin. EMBO J. 1985 Dec 16;4(13A):3393-8. (DECMA-1, IP, WB, FA, PubMed)

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