Product Information

Contents: Affinity Purified anti-mouse PDCA-1 (plasmacytoid DC Ag-1, BST2, CD317)
Catalog Number: 14-3172
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio927
Isotype: Rat IgG2b

Staining of BST2 transfected cells with 0.06 μg of Purified anti-mouse PDCA-1 (plasmacytoid DC Ag-1, BST2, CD317) followed by PE Anti-Rat IgG (cat. 12-4822). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-3172	Affinity Purified anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	N/A	N/A	FC
16-3172	Functional Grade* Purified anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	N/A	N/A	FA FC
13-3172	Biotin anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	N/A	N/A	FC
11-3172	Fluorescein isothiocyanate (FITC) anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	488	518	FC
12-3172	Phycoerythrin (PE) anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	488	575	FC
51-3172	Alexa Fluor® 647 anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	633	668	FC
53-3172	Alexa Fluor® 488 anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	488	519	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naïve mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8.

The eBio927 monoclonal antibody has also been shown to have functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify and identify pDCs.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio927 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio927 antibody has been tested by flow cytometric analysis of BST2 transfected cells. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For staining of endogenous PDCA-1 in tissue/cells, it is preferrable to use direct conjugates rather than two-step staining with purified antibody.

References

Blasius A.L., E. Giurisat, M. Cella, R.D. Schreiber, A.S. Shaw, M. Colonna. 2006. Bone Marrow Stromal Cell Antigen 2 Is a Specific Marker of Type I IFN-Producing Cells in the Naïve Mouse, but a Promiscuous Cell Surface Antigen Following IFN Stimulation. J Immunol. 177:3260-3265 (927, FC, FA)

Related Products

Cat. 14-4031  Purified Rat IgG2b Isotype Control
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 11-4811  FITC Anti-Rat IgG
Cat. 13-4813  Biotin Anti-Rat IgG (clone Polyclonal)