Product Information

Contents: Affinity Purified anti-human CD277 (B7 family, Butyrophilin)
Catalog Number: 14-2779
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioBT3.1 (20.1, BT3.1)
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with .5 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or .5 μg of Purified anti-human CD277 (eBioBT3.1) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-2779	Affinity Purified anti-human CD277 (B7 family, Butyrophilin)	N/A	N/A	FC IP
13-2779	Biotin anti-human CD277 (B7 family, Butyrophilin)	N/A	N/A	FC
12-2779	Phycoerythrin (PE) anti-human CD277 (B7 family, Butyrophilin)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioBT3.1 monoclonal antibody recognizes BT3.1, BT3.2 and BT3.3. The BT family of proteins is a subgroup of the Ig superfamily (IgSF). BT is a glycoprotein that forms a major component of the milk fat globule molecule. In addition to BT, there are six other genes that have been discovered which have been sub-divided into the BT2 (BT2.1, BT2.2, and BT2.3) and BT3 (BT3.1, BT3.2, and BT3.3) families. BT3.1, BT3.2, and BT3.3 share 95% mRNA identity. BT3 molecules are constitutively expressed on the cell surface of antigen-presenting cells, and the IgV-like domain of BT3.1 is sufficient for interaction with its counter-receptor. BT3.1 is expressed by T cells, B cells and CD14+ cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioBT3.1 (20.1, BT3.1) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested

This eBioBT3.1 (20.1, BT3.1) antibody has been tested by flow cytometric analysis of normal human peripheral blood mononuclear cells. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Compte, E., P. Pontarotti, Y. Collette, M. Lopez, and D. Olive. 2004. Characterization of BT3 molecules belonging to the B7 family expressed on immune cells. Eur. J. Immunol. 34: 2089-2099. (BT3.1, FC, WB, PubMed)

Tazi-Ahnini, R., J. Henry, C. Offer, C. Bouissou-Bouchouata, I.H. Mather, and P. Pontarotti. 1997. Cloning, localization, and structure of new members of the butyrophilin gene family in the juxta-telomeric region of the major histocompatibility complex. Immunogenetics. 47: 55-63.

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