Product Information

Contents: Affinity Purified anti-human CD244 (2B4, p38)
Catalog Number: 14-2449
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioPP35 (PP35)
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with APC anti-human CD8a (RPA-T8) (cat. 17-0088) and 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (left) or 0.25 μg of Purified anti-human CD244 (PP35) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-2449	Affinity Purified anti-human CD244 (2B4, p38)	N/A	N/A	FC IP WB
16-2449	Functional Grade* Purified anti-human CD244 (2B4, p38)	N/A	N/A	FA
11-2449	Fluorescein isothiocyanate (FITC) anti-human CD244 (2B4, p38)	488	518	FC
17-2449	Allophycocyanin (APC) anti-human CD244 (2B4, p38)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioPP35 monoclonal antibody reacts with human CD244, also known as 2B4, p38, NK-cell activation-inducing ligand, signaling lymphocyte activation molecule family member 4, and natural killer cell receptor 2B4. Human CD244 is a 38 kD protein expressed on NK cells, a subset of CD8+ T cells, γδ T cells, monocytes, basophils, mast cells, and eosinophils. Binding of CD244 to its ligand, CD48, results in NK cell activation. Costaining experiments with eBioC1.7 suggest that both antibodies recognize different epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioPP35 (PP35) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).

References

Moretta A, Bottino C, Tripodi G, Vitale M, Pende D, Morelli L, Augugliaro R, Barbaresi M, Ciccone E, Millo R, et al. Novel surface molecules involved in human NK cell activation and triggering of the lytic machinery. Int J Cancer Suppl. 1992;7:6-10. (PP35, mAb development, FA, PubMed)

Parolini S, Bottino C, Falco M, Augugliaro R, Giliani S, Franceschini R, Ochs HD, Wolf H, Bonnefoy JY, Biassoni R, Moretta L, Notarangelo LD, Moretta A. X-linked lymphoproliferative disease. 2B4 molecules displaying inhibitory rather than activating function are responsible for the inability of natural killer cells to kill Epstein-Barr virus-infected cells. J Exp Med. 2000 Aug 7;192(3):337-46. (PP35, FC, FA, PubMed)

Sivori S, Falco M, Marcenaro E, Parolini S, Biassoni R, Bottino C, Moretta L, Moretta A. Early expression of triggering receptors and regulatory role of 2B4 in human natural killer cell precursors undergoing in vitro differentiation. Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4526-31. (PP35, FC, PubMed)

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