human CD244 (2B4, p38) antibody: purified antibody

Contents: Affinity Purified anti-human CD244 (2B4, p38)
Catalog Number: 14-2449
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioPP35 (PP35)
Isotype: Mouse IgG1

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-2449-80	25 ug	$65
14-2449-82	100 ug	$105

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with APC anti-human CD8a (RPA-T8) (cat. 17-0088) and 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (left) or 0.25 μg of Purified anti-human CD244 (PP35) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-2449	Fluorescein isothiocyanate (FITC) anti-human CD244 (2B4, p38)	488	518	FC
14-2449	Affinity Purified anti-human CD244 (2B4, p38)	N/A	N/A	FC IP WB
16-2449	Functional Grade* Purified anti-human CD244 (2B4, p38)	N/A	N/A	FA
17-2449	Allophycocyanin (APC) anti-human CD244 (2B4, p38)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioPP35 monoclonal antibody reacts with human CD244, also known as 2B4, p38, NK-cell activation-inducing ligand, signaling lymphocyte activation molecule family member 4, and natural killer cell receptor 2B4. Human CD244 is a 38 kD protein expressed on NK cells, a subset of CD8+ T cells, γδ T cells, monocytes, basophils, mast cells, and eosinophils. Binding of CD244 to its ligand, CD48, results in NK cell activation. Costaining experiments with eBioC1.7 suggest that both antibodies recognize different epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioPP35 (PP35) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).

References

Moretta A, Bottino C, Tripodi G, Vitale M, Pende D, Morelli L, Augugliaro R, Barbaresi M, Ciccone E, Millo R, et al. Novel surface molecules involved in human NK cell activation and triggering of the lytic machinery. Int J Cancer Suppl. 1992;7:6-10. (PP35, mAb development, FA, PubMed)

Parolini S, Bottino C, Falco M, Augugliaro R, Giliani S, Franceschini R, Ochs HD, Wolf H, Bonnefoy JY, Biassoni R, Moretta L, Notarangelo LD, Moretta A. X-linked lymphoproliferative disease. 2B4 molecules displaying inhibitory rather than activating function are responsible for the inability of natural killer cells to kill Epstein-Barr virus-infected cells. J Exp Med. 2000 Aug 7;192(3):337-46. (PP35, FC, FA, PubMed)

Sivori S, Falco M, Marcenaro E, Parolini S, Biassoni R, Bottino C, Moretta L, Moretta A. Early expression of triggering receptors and regulatory role of 2B4 in human natural killer cell precursors undergoing in vitro differentiation. Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4526-31. (PP35, FC, PubMed)

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control
Cat. 14-5838    Affinity Purified anti-human CD244 (2B4, p38) (clone eBioC1.7 (C1.7))