Product Information

Contents: Affinity Purified anti-human CRACC (CD2 like receptor activating cytotoxic cells, CD319, CS1, 19A24)
Catalog Number: 14-2229
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 162
Isotype: Mouse IgG2b, κ

Staining of normal human peripheral blood cells with PE anti-human p75/AIRM1 (QA79) (cat. 12-5759) and 1 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (left) or 1 μg of Purified anti-human CRACC (162) (right) followed by Biotin Anti-Mouse IgG (cat. 13-4013) and SaV-APC (cat. 17-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-2229	Affinity Purified anti-human CRACC (CD2 like receptor activating cytotoxic cells, CD319, CS1, 19A24)	N/A	N/A	FC IP
12-2229	Phycoerythrin (PE) anti-human CRACC (CD2 like receptor activating cytotoxic cells, CD319, CS1, 19A24)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody 162 recognizes CRACC (CD2 like receptor for activating cytotoxic cells) also known as CD319 and CS1. It is a member of the SLAM receptor family. CRACC is a 66kDa glycosylated transmembrane protein which contains a cytoplasmic ITSM domain (Immunoreceptor Tyrosine-based Switch Motif). It is this domain, found in other proteins such as CD244 and NTB-A, that interacts with SH2 containing proteins such as SAP. Interestingly the ITSM domain in CRACC is SAP independent. Instead EAT-2 acts as the signaling molecule for CRACC. This has profound impact for patients with X-linked lymphoproliferative disease (contains a SAP mutation). It should be noted that a splice variant has been reported which deletes the ITSM domain.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 162 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested

This 162 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Bouchon A, Cella M, Grierson HL, Cohen JI, Colonna M. Activation of NK cell-mediated cytotoxicity by a SAP-independent receptor of the CD2 family. J Immunol. 2001 Nov 15;167(10):5517-21. (162, FC, FA, IP PubMed)

Tassi I, Colonna M. The cytotoxicity receptor CRACC (CS-1) recruits EAT-2 and activates the PI3K and phospholipase Cgamma signaling pathways in human NK cells. J Immunol. 2005 Dec 15;175(12):7996-8002. {162, FA PubMed)

Veillette A. NK cell regulation by SLAM family receptors and SAP-related adapters. Immunol Rev. 2006 Dec;214:22-34. Review.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4732  Affinity Purified Mouse IgG2b Isotype Control