Product Information

Contents: Affinity Purified anti-mouse CIRE (DC-SIGN, CD209)
Catalog Number: 14-2091
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 5H10
Isotype: Rat IgG2a

CIRE/DC-SIGN-transfected (left) and untransfected control CHO lysates were loaded at 1x10⁵ cells/lane, probed with 1 µg/mL purified 5H10 and revealed with HRP anti-rat IgG.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-2091	Affinity Purified anti-mouse CIRE (DC-SIGN, CD209)	N/A	N/A	IP WB
13-2091	Biotin anti-mouse CIRE (DC-SIGN, CD209)	N/A	N/A	FC
12-2091	Phycoerythrin (PE) anti-mouse CIRE (DC-SIGN, CD209)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 5H10 antibody was generated by immunization with a peptide derived from the extracellular region of mouse CIRE/DC-SIGN (CD209). CIRE/DC-SIGN was identified by its expression on CD8α- dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN. Human DC-SIGN was originally identified in human placenta for its ability to bind the HIV envelope protein gp120 in a CD4-independent manner. CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein. It contains a C-terminal, extracellular, Carbohydrate Recognition Domain (CRD) that is predicted to bind mannose and other carbohydrates in a Ca²⁺ dependent manner. It has been postulated that CIRE/DC-SIGN may play a role in T-dendritic cell interactions through binding with members of the ICAM family. CIRE/DC-SIGN is differentially expressed by sub-populations of dendritic cells and preliminary data suggest that its expression varies depending on the activation state of the host. CIRE/DC-SIGN is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. While human DC-SIGN is predominantly expressed in dendritic cells, CIRE/DC-SIGN mRNA has also been detected in B cells. The 5H10 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 5H10 antibody has been reported for use in immunoprecipitation, and immunoblotting (WB).

Applications Tested

This 5H10 antibody has been tested by immunoblotting and immunoprecipitation of CIRE in CIRE-transfected CHO cells. The reactivity of this antibody has been confirmed by immunoprecipitation of CIRE with 5H10 followed by immunoblotting with another CIRE-specific monoclonal antibody, LWC06 (cat. 14-2092) and, immunoprecipitation of CIRE with LWC06 followed by immunoblotting with 5H10.

References

Caminschi I, Lucas KM, O’Keeffe MA, Hochrein H, Laabi Y, Brodnicki TC, Lew AM, Shortman K, and Wright MD. 2001. Molecular cloning of a C-type lectin superfamily protein differentially expressed by CD8α^- splenic dendritic cells. Mol Immunol. 38: 365-373.

O’Keeffe M, Hochrein H, Vremec D, Caminschi I, Miller JL, Anders EM, Wu L, Lahoud MH, Henri S, Scott B, Hertzog P, Tatarczuch L, and Shortman K. 2002. Mouse plasmacytoid cells: long-lived cells, heterogeneous in surface phenotype and function, that differentiate into CD8⁺ dendritic cells only after microbial stimulus. J Exp Med. 196(10): 1307-1319. (5H10, FC, PubMed)

Park CG, Takahara K, Umemoto E, Yashima Y, Matsubara K, Matsuda Y, Clausen BE, Inaba K, and Steinman RM. 2001. Five mouse homologues of the human dendritic cell C-type lectin, DC-SIGN. International Immunology. 13(10): 1283-1290.

Caminschi I, Corbett AJ, Zahra C, Lahoud M, Lucas KM, Sofi M, Vremec D, Gramberg T, Pohlmann S, Curtis J, Handman E, van Dommelen SL, Fleming P, Degli-Esposti MA, Shortman K, Wright MD. Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN. Int Immunol. 2006 May;18(5):741-53. (5H10, FC, PubMed)

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