Product Information

Contents: Affinity Purified anti-mouse CD207 (Langerin)
Catalog Number: 14-2075
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioL31
Isotype: Rat IgG2a

Mouse CD207-transfected (Lane 2) and untransfected control 293T (Lane 1) lysates were immunoblotted with 1 µg/mL purified eBioL31 and revealed with HRP anti-rat IgG.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-2075	Affinity Purified anti-mouse CD207 (Langerin)	N/A	N/A	FC IC Flow IHC IP WB
13-2075	Biotin anti-mouse CD207 (Langerin)	N/A	N/A	IC Flow
12-2075	Phycoerythrin (PE) anti-mouse CD207 (Langerin)	488	575	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioL31 is a monoclonal antibody that reacts with mouse Langerin (CD207) which is expressed in a distinct subset of dendritic cells called Langerhans cells (LC). Mouse Langerin is a 48kDa C-type lectin transmembrane protein that likely plays a role in antigen recognition and uptake. Langerhans cells are located in the epidermis and upon activation, reduce Langerin expression and begin migrating through the dermis towards lymphatic vessels. Expression of mouse Langerin in LC has been correlated to the presence of Birbeck Granules. Furthermore, the formation of Birbeck Granules has been observed upon transfection of Langerin cDNA into fibroblast cell lines.

Western Blotting with eBioL31 reveals a 48kDa band in lysate from mouse ear epidermis, as well as in lysates from cells transfected with mouse Langerin cDNA. Preliminary data from mouse Langerin cDNA transfected cells suggests that eBioL31 can be used for intracellular flow cytometric analysis in combination with the Foxp3 bufer set (cat 00-5523). The epitope recognized by eBioL31 is in the extracellular domain within the CRD (carbohydrate recognition domain) and therefore can be used to detect both surface and intracellular langerin. Differences in expression has been observed between mouse strains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioL31 antibody has been reported for use in flow cytometric analysis, intracellular staining followed by flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining (electron microscopy). (Fluorochrome conjugated eBioL31 is recommended for use in flow cytometry.)

Applications Tested

This eBioL31 antibody has been tested by flow cytometric analysis of transfected cells and western blotting or mouse ear lysates. This can be used at less than or equal to 1 μg/ml for immunoblotting. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Idoyaga J, Suda N, Suda K, Park CG, Steinman RM. Antibody to Langerin/CD207 localizes large numbers of CD8alpha+ dendritic cells to the marginal zone of mouse spleen. Proc Natl Acad Sci U S A. 2009 Feb 3;106(5):1524-9. (L31, FC, IHC frozen sections)

Cheong C, Idoyaga J, Do Y, Pack M, Park SH, Lee H, Kang YS, Choi JH, Kim JY, Bonito A, Inaba K, Yamazaki S, Steinman RM, Park CG. Production of monoclonal antibodies that recognize the extracellular domain of mouse Langerin/CD207. J Immunol Methods. 2008 Feb 29;331(1-2):27-38. (eBioL31, FC, IC, IP, IHC, WB PubMed)

Stoitzner P, Holzman , McLellan AD, Ivarsson L, Stossel H, Kapp M, Kammerer U, Douillard P, Kampgen E, Koch F, Saeland S, Romani N. Visualization and characterization of migratory Langerhans cells in murine skin and lymph nodes by antibodies against Langerin/CD207. J Invest Dermatol. 2003 Feb;120(2):266-74.

Valladeau J, Clair-Moninot V, Dezutter-Dambuyant C, Pin JJ, Kissenpfennig A, Mattei MG, Ait-Yahia S, Bates EE, Malissen B, Koch F, Fossiez F, Romani N, Lebecque S, Saeland S. Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. J Immunol. 2002 Jan 15;168(2):782-92.

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