Contents: Affinity Purified anti-mouse CD207 (Langerin)
Catalog Number: 14-2073
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioRMUL.2
Isotype: Rat IgG2a, κ
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WB left: Lysate from C57/Bl6 mouse ear epidermis (left), CD207-transfected (middle) and untransfected 293T (right) were probed with 2 µg/mL purified eBioRMUL.2 and revealed with HRP anti-rat IgG.
Right: Staining of mouse epidermal sheets using RMUL.2.(top) or rat IgG2a (14-4321)(bottom). Courtesy Mark Udey and Chris Nagao.
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Questions? Please consult our answers to frequently asked questions at
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Description
RMUL.2 is a monoclonal antibody that reacts with the predicted extracellular domain of mouse Langerin (CD207). Mouse Langerin(CD207) is expressed in a distinct subset of Dendritic cells called Langerhans cells (LC). Mouse Langerin is a 48kDa C-type lectin putative transmembrane protein that likely plays a role in antigen recognition and uptake. Langerhans cells are located in the epidermis and upon activation, reduce Langerin expression and begin migrating through the dermis towards lymphatic vessels. Expression of mouse Langerin in LC has been correlated to the presence of Birbeck Granules. Furthermore, the formation of Birbeck Granules has been observed upon transfection of Langerin cDNA into fibroblast cell lines.
Western Blotting with RMUL.2 reveals a 48kDa band in lysate from mouse ear epidermis, as well as in lysates from cells transfected with mouse Langerin cDNA. Cross reactivity of this antibody with other proteins has not been determined. Preliminary data from mouse Langerin cDNA transfected cells suggests that RMUL.2 can be used for intracellular flow cytometric analysis. We have observed in our transfectants the majority of protein is expressed intracellularly with a small percentage on the cell surface. In addition, RMUL.2 has also been tested by immunohistology on mouse ear epidermal sheets and shows specific staining when compared to a polyclonal antibody obtained from a different supplier.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioRMUL.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. (Fluorochrome conjugated eBioRMUL.2 is recommended for use in intracellular flow cytometry.)
Applications Tested
This eBioRMUL.2 antibody has been tested by immunoblotting. This can be used at less than or equal to 2μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Stoitzner P, Holzman , McLellan AD, Ivarsson L, Stossel H, Kapp M,
Kammerer U, Douillard P, Kampgen E, Koch F, Saeland S, Romani N. Visualization and characterization of migratory Langerhans cells in murine skin and lymph nodes by antibodies against Langerin/CD207.
J Invest Dermatol. 2003 Feb;120(2):266-74. .
Valladeau J, Clair-Moninot V, Dezutter-Dambuyant C, Pin JJ, Kissenpfennig A, Mattei MG, Ait-Yahia S, Bates EE, Malissen B, Koch F, Fossiez F, Romani N, Lebecque S, Saeland S. Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues.
J Immunol. 2002 Jan 15;168(2):782-92.
Molinero LL, Zhou P, et al. 2008. Epidermal Langerhans cells promote skin allograft rejection in mice with NF-kappa B-impaired T cells. Am J Transplant. 8(1):21-31. (
eBioRMUL.2, IC flow,
PubMed)
Related Products
Cat. 14-4321 Affinity Purified Rat IgG2a Isotype Control
Mouse Cellular Antigens Chart