Product Information

Contents: Affinity Purified anti-human CD205 (DEC-205)
Catalog Number: 14-2059
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: MG38
Isotype: Mouse IgG2b, κ
HLDA No.: N/A

Staining of normal human peripheral blood lymphocytes (left) and mature dendritic cells (right) with 0.125 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (open histogram) or 0.015 μg of Purified MG38 (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013) and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-2059	Affinity Purified anti-human CD205 (DEC-205)	N/A	N/A	FC IH/F IP
13-2059	Biotin anti-human CD205 (DEC-205)	N/A	N/A	FC
11-2059	Fluorescein (FITC) anti-human CD205 (DEC-205)	488	518	FC
12-2059	Phycoerythrin (PE) anti-human CD205 (DEC-205)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MG38 monoclonal antibody reacts with human CD205, also known as DEC-205, an integral membrane protein homologous to the macrophage mannose receptor. CD205 is an endocytic dendritic receptor that mediates efficient processing and presentation of antigens on MHC class II productions in vivo. CD205 is highly expressed by dendritic cells (DCs) within the T cell areas of lymphoid tissues, particularly on CD8⁺ DCs that are believed to play a role in cross-priming. Expression of CD205 is detected with this antibody at high intensity on in vitro derived mature human DCs (mDC) and at intermediate intensity on immature human DCs (iDC). All leukocytes from peripheral blood show a degree of staining with this mAb that can be minimized by using very low concentrations of MG38.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MG38 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.

Applications Tested

This MG38 antibody has been tested by flow cytometric analysis of in vitro derived human dendritic cells and peripheral blood leukocytes. This can be used at less than or equal to 0.03 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hawiger D, Inaba K, Dorsett Y, Guo M, Mahnke K, Rivera M, Ravetch JV, Steinman RM, Nussenzweig MC. (2001) Dendritic cells induce peripheral T cell unresponsiveness under steady state conditions in vivo. J Exp Med. Sep 17;194(6):769-79.

Bonifaz L, Bonnyay D, Mahnke K, Rivera M, Nussenzweig MC, Steinman RM. (2002) Efficient targeting of protein antigen to the dendritic cell receptor DEC-205 in the steady state leads to antigen presentation on major histocompatibility complex class I products and peripheral CD8+ T cell tolerance. J Exp Med. Dec 16;196(12):1627-38.

Jiang W, Swiggard WJ, Heufler C, Peng M, Mirza A, Steinman RM, Nussenzweig MC. (1995) The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing. Nature. May 11;375(6527):151-5.

Guo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM. 2000. A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum Immunol. 61(8):729-38.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4732  Affinity Purified Mouse IgG2b Isotype Control