mouse CCR9 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-mouse CD199 (CCR9)
Catalog Number: 14-1991
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioCW-1.2 (CW-1.2)
Isotype: Mouse IgG2a

Product Options		Price
Cat. No.	Size	To view relevant pricing, please select your country and resume browsing.
14-1991-80	25 ug
14-1991-82	100 ug
14-1991-85	500 ug

Staining of C57Bl/6 thymocytes with 0.25 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (open histogram) or 0.25 μg of Purified anti-mouse CCR9 (CW-1.2) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1991	Affinity Purified anti-mouse CD199 (CCR9)	N/A	N/A	FC
13-1991	Biotin anti-mouse CD199 (CCR9)	N/A	N/A	FC
11-1991	Fluorescein isothiocyanate (FITC) anti-mouse CD199 (CCR9)	488	518	FC
12-1991	Phycoerythrin (PE) anti-mouse CD199 (CCR9 )	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioCW-1.2 monoclonal antibody reacts with mouse CCR9 (CDw199), which is the receptor for thymus-expressed chemokine (TECK). CCR9 is a member of the G protein coupled receptor (GPCR) supergene family, and is involved in trafficking of T cell progenitors within the thymus. CCR9 expression during thymocyte development commences at the double-negative (DN) 3 stage (CD4-CD8-CD25+CD44-), peaks in the double-positive (DP) stage (CD4+CD8+CD25-CD44-), and is down-regulated in commited CD4+ or CD8+ single-positive (SP) thymoyctes. CCR9-deficient mice show a mild impairment in thymoycte development. In the periphery, CCR9 is thought to be expressed on naïve CD8+ T cells, but not on naïve CD4+ T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioCW-1.2 (CW-1.2) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioCW-1.2 (CW-1.2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Zaballos A, Gutierrez J, Varona R, Ardavin C, Marquez G. Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. J Immunol. 1999 May 15;162(10):5671-5.

Wurbel MA, Malissen B, Campbell JJ. Complex regulation of CCR9 at multiple discrete stages of T cell development. Eur J Immunol. 2006 Jan;36(1):73-81. (CW-1.2, FC, Development of mAb, PubMed)

Liu C, Saito F, Liu Z, Lei Y, Uehara S, Love P, Lipp M, Kondo S, Manley N, Takahama Y. Coordination between CCR7- and CCR9-mediated chemokine signals in pre-vascular fetal thymus colonization. Blood. 2006 Jun 29.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4724  Affinity Purified Mouse IgG2a, K Isotype Control