Immunoblotting of 106 normal peripheral blood cell lysates with 5 µg/mL of Anti-Human CD196 (CCR6) Purified. The band was visualized using Anti-Mouse IgG HRP.
Contents: Anti-Human CD196 (CCR6) Purified Catalog Number: 14-1969 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Clone: R6H1 Host/Isotype: Mouse IgG1
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Size
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14-1969-80
25 ug
14-1969-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
This R6H1 monoclonal antibody reacts with CD196 (also known as CCR6), a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, natural killer, and Langerhans cells. This CC chemokine receptor uniquely binds MIP-3a/CCL20.
A chemoattractant for dendritic cells, effector/memory T cells, and B cells, CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This R6H1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunoprecipitation.
Applications Tested
This R6H1 antibody has been tested by western blot on lysates prepared from normal human peripheral blood cells. This antibody can be used at less than or equal to 5 ug/mL.
References
Liu H, Rohowsky-Kochan C.Regulation of IL-17 in human CCR6+ effector memory T cells. J Immunol. 2008 Jun 15;180(12):7948-57
Singh SP, Zhang HH, Foley JF, Hedrick MN, Farber JM.Human T cells that are able to produce IL-17 express the chemokine receptor CCR6. J Immunol. 2008 Jan 1;180(1):214-21
Lim HW, Lee J, Hillsamer P, Kim CH.Human Th17 cells share major trafficking receptors with both polarized effector T cells and FOXP3+ regulatory T cells. J Immunol. 2008 Jan 1;180(1):122-9
Shutyser E, Struyf S, Van Damme J.The CC chemokine CCL20 and its receptor CCR6. Cytokine Growth Factor Rev. 2003 Oct;14(5):409-26.
Carramolino L, Kremer L, Goya I, Varona R, Buesa JM, Gutiérrez J, Zaballos A, Martínez-A C, Márquez G. Down-regulation of the beta-chemokine receptor CCR6 in dendritic cells mediated by TNF-alpha and IL-4. J Leukoc Biol. 1999 Nov;66(5):837-44. (clone R6H1, FC, Pubmed)
