14-1958

Print Page

Adobe PDF

Contents: Affinity Purified anti-human CCR5 (CD195)
Catalog Number: 14-1958
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: R22/7
Isotype: Mouse IgG1, κ
HLDA No.: N/A

Product Options

Price

Cat. No.

Size

To view relevant pricing,
please select your country
and resume browsing.

Staining of normal human peripheral blood cells with 1.0 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 1.0 μg of Purified anti-human CCR5 (R22/7) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-1958	Phycoerythrin (PE) anti-human CCR5 (CD195)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The R22/7 monoclonal antibody reacts with the amino terminus of human CCR5 (CD195), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1a, MIP-1ß and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The R22/7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and ELISA.

Applications Tested

This R22/7 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Special Notes

R22/7 stains with lower intensity on human peripheral blood mononuclear cells, than on human lysed whole blood. For staining human peripheral blood mononuclear cells, we recommend the use of clone eBioT21/8 (cat. 1957).

References

Pollok-Kopp B, Schwarze K, Baradari VK, Oppermann M. Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8. (R22/7, WB, IP, PubMed)

Deng H, Liu R, Ellmeier W, Choe S, Unutmaz D, Burkhart M, Di Marzio P, Marmon S, Sutton RE, Hill CM, Davis CB, Peiper SC, Schall TJ, Littman DR, Landau NR. Identification of a major co-receptor for primary isolates of HIV-1. Nature. 1996 Jun 20;381(6584):661-6.

Raport CJ, Gosling J, Schweickart VL, Gray PW, Charo IF. Molecular cloning and functional characterization of a novel human CC chemokine receptor (CCR5) for RANTES, MIP-1beta, and MIP-1alpha. J Biol Chem. 1996 Jul 19;271(29):17161-6.

Related Products

Cat. 00-0000    eBioscience 10X ELISA Coating Buffer
Cat. 44-2404    Nunc MaxiSorp™ flat-bottom 96 well plate
Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 18-4100    Avidin Horse Radish Peroxidase (AV-HRP)
Cat. 00-4202    eBioscience ELISA Diluent Solution, 5X
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control
Cat. 18-8877    See Catalog # 18-8817 for an alternative product: Mouse TrueBlot™: Horseradish Peroxidase (HRP) anti-mouse IgG