Product Information

Contents: Affinity Purified anti-human CCR5 (CD195)
Catalog Number: 14-1957
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioT21/8 (T21/8)
Isotype: Mouse IgG1, κ

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CCR5 (T21/8) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1957	Affinity Purified anti-human CCR5 (CD195)	N/A	N/A	ELISA FC IHC IP WB
13-1957	Biotin anti-human CCR5 (CD195)	N/A	N/A	FC IHC
12-1957	Phycoerythrin (PE) anti-human CCR5 (CD195)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioT21/8 monoclonal antibody reacts with the amino terminus of human CCR5 (CD195), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1α, MIP-1β and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sub-optimal staining conditions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioT21/8 (T21/8) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) and immunohistochemical staining. The eBioT21/8 monoclonal antibody has also been used as the capture antibody for the ELISA detection of human CCR5 in cell lysates.

Applications Tested

This eBioT21/8 (T21/8) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Special Notes

Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sup-optimal staining conditions. Therefore, staining conditions should be closely monitored to ensure consistent staining.

References

Pollok-Kopp B, Schwarze K, Baradari VK, Oppermann M. Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8. (eBioT21/8, ELISA, IHC, PubMed)

Deng H, Liu R, Ellmeier W, Choe S, Unutmaz D, Burkhart M, Di Marzio P, Marmon S, Sutton RE, Hill CM, Davis CB, Peiper SC, Schall TJ, Littman DR, Landau NR. Identification of a major co-receptor for primary isolates of HIV-1. Nature. 1996 Jun 20;381(6584):661-6.

Raport CJ, Gosling J, Schweickart VL, Gray PW, Charo IF. Molecular cloning and functional characterization of a novel human CC chemokine receptor (CCR5) for RANTES, MIP-1beta, and MIP-1alpha. J Biol Chem. 1996 Jul 19;271(29):17161-6.

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