Affinity Purified anti-human CCR3 (CD193, CKR3)

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Contents: Affinity Purified anti- human CD193 (CCR3, CKR3)
Catalog Number: 14-1939
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio5E8-G9-B4 (5E8-G9-B4)
Isotype: Mouse IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-1939-80	25 ug	$85
14-1939-82	100 ug	$185

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with 0.5 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (left) or 0.5 μg of Purified anti-human CD193 (5E8-G9-B4) (right) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1939	Affinity Purified anti-human CCR3 (CD193, CKR3)	N/A	N/A	FC
17-1939	Allophycocyanin (APC) anti-human CCR3 (CD193, CKR3)	633	660	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio5E8-G9-B4 monoclonal antibody reacts with human CD193 (CCR3, CKR3). CD193 is a member of the seven transmembrane G-protein coupled receptor (GPCR) family, and is a high affinity chemokine receptor for the chemokines eotaxin-1 (CCL11), eotaxin-2 (CCL24), eotaxin-3 (CCL26) and MCP-4 (CCL13), but has also been reported to bind RANTES, MCP-3 and MCP-4. CD193 is highly expressed on the surface of eosinophils, and is the key eosinophil chemokine receptor responsible for the regulation of eosinophil migration and function. In addition to eosinophils, CD193 is also expressed on CD4+ TH2 cells, basophils, mast cells, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitors and airway epithelial cells. CD193 likely plays an important role in the migration of eosinophils in allergic airway inflammation and asthma. CD193 can also serve as a receptor for HIV-1 cell entry.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio5E8-G9-B4 (5E8-G9-B4) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio5E8-G9-B4 (5E8-G9-B4) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Choe H, Farzan M, Sun Y, Sullivan N, Rollins B, Ponath PD, Wu L, Mackay CR, LaRosa G, Newman W, Gerard N, Gerard C, Sodroski J. The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell. 1996 Jun 28;85(7):1135-48.

Heath H, Qin S, Rao P, Wu L, LaRosa G, Kassam N, Ponath PD, Mackay CR. Chemokine receptor usage by human eosinophils. The importance of CCR3 demonstrated using an antagonistic monoclonal antibody. J Clin Invest. 1997 Jan 15;99(2):178-84.

Forssmann U, Uguccioni M, Loetscher P, Dahinden CA, Langen H, Thelen M, Baggiolini M. Eotaxin-2, a novel CC chemokine that is selective for the chemokine receptor CCR3, and acts like eotaxin on human eosinophil and basophil leukocytes. J Exp Med. 1997 Jun 16;185(12):2171-6.

Sallusto F, Mackay CR, Lanzavecchia A. Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells. Science. 1997 Sep 26;277(5334):2005-7.

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