Product Information

Contents: Affinity Purified anti-human CD181 (CXCR1, IL-8RA)
Catalog Number: 14-1819
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio8F1-1-4 (8F1-1-4)
Isotype: Mouse IgG2b, κ

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (left) or 0.25 μg of Purified anti-human CD181 (8F1-1-4) (right) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1819	Affinity Purified anti-human CD181 (CXCR1, IL-8RA, IL8RA)	N/A	N/A	FC IHC
11-1819	Fluorescein isothiocyanate (FITC) anti-human CD181 (CXCR1, IL-8RA, IL8RA)	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio8F1-1-4 monoclonal antibody reacts with human CD181 (CXCR1, IL-8Rα). CD181 is a 67-70 kDa member of the 7-transmembrane spanning G-protein coupled receptor (GPCR) family. CD181 is expressed as a homodimer, or a heterodimer with CD182 (CXCR2, IL-8Rβ) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO-α, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio8F1-1-4 (8F1-1-4) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested

This eBio8F1-1-4 (8F1-1-4) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Wolf M, Delgado MB, Jones SA, Dewald B, Clark-Lewis I, Baggiolini M. Granulocyte chemotactic protein 2 acts via both IL-8 receptors, CXCR1 and CXCR2. Eur J Immunol. 1998 Jan;28(1):164-70.

Lippert U, Artuc M, Grutzkau A, Moller A, Kenderessy-Szabo A, Schadendorf D, Norgauer J, Hartmann K, Schweitzer-Stenner R, Zuberbier T, Henz BM, Kruger-Krasagakes S. Expression and functional activity of the IL-8 receptor type CXCR1 and CXCR2 on human mast cells. J Immunol. 1998 Sep 1;161(5):2600-8.

Li F, Zhang X, Gordon JR. CXCL8((3-73))K11R/G31P antagonizes ligand binding to the neutrophil CXCR1 and CXCR2 receptors and cellular responses to CXCL8/IL-8. Biochem Biophys Res Commun. 2002 May 10;293(3):939-44.

Sprenger H, Lloyd AR, Meyer RG, Johnston JA, Kelvin DJ. Genomic structure, characterization, and identification of the promoter of the human IL-8 receptor A gene. J Immunol. 1994 Sep 15;153(6):2524-32.

Chuntharapai A, Lee J, Hebert CA, Kim KJ. Monoclonal antibodies detect different distribution patterns of IL-8 receptor A and IL-8 receptor B on human peripheral blood leukocytes. J Immunol. 1994 Dec 15;153(12):5682-8.

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