Product Information

Contents: Affinity Purified anti-mouse CD180 (RP105, Toll-like Receptor/ TLR Family)
Catalog Number: 14-1801
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: RP/14
Isotype: Rat IgG2a, κ

Surface staining of mouse splenocytes with anti-mouse CD180 (RP/14) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1801	Affinity Purified anti-mouse CD180 (RP105, Toll-like Receptor/ TLR Family)	N/A	N/A	FA FC IP
16-1801	Functional Grade* Purified anti-mouse CD180 (RP105, Toll-like Receptor/ TLR Family)	N/A	N/A	FA FC
13-1801	Biotin anti-mouse CD180 (RP105, Toll-like Receptor/ TLR Family)	N/A	N/A	FC
12-1801	PE anti-mouse CD180 (RP105, Toll-like Receptor/ TLR Family)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RP/14 monoclonal antibody reacts with mouse CD180 (RP105). This 105 kDa type I transmembrane molecule is a member of the TLR family of proteins characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. RP105 physically associates with another molecule called MD-1 and is expressed on mature B cells, monocytes/macrophages and dendritic cells. Ligation of RP105 with RP/14 protects B cells from irradiation- or dexamethasone-induced apoptosis, and drives them to proliferate. B cells activated by RP105-ligation arrest their growth and undergo apoptosis when the antigen receptor is engaged. The RP105/MD-1 complex in concert with TLR4 mediates B cell recognition and signaling of LPS. Thus, RP105 is a signal transduction molecule and plays a role in regulation of B cell growth and death.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RP/14 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported in B cell activation in functional studies. (Please use Functional Grade purified RP/14, cat. 16-1801, in functional assays.)

Applications Tested

The RP/14 antibody has been tested by flow cytometric analysis of mouse spelnocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Miyake, K., Y. Yamashita, et al. 1995. RP105, a novel B cell surface molecule implicated in B cell activation, is a member of the leucine-rich repeat protein family. J Immunol 154(7): 3333-40.
Miyake, K., R. Shimazu, et al. 1998. Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression. J Immunol 161(3): 1348-53.
Ogata, H., I. Su, et al. 2000. The toll-like receptor protein RP105 regulates lipopolysaccharide signaling in B cells. J Exp Med 192(1): 23-9.

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