Product Information

Contents: Affinity Purified anti-human CD171 (Neurite Cell Adhesion Molecule L1, L1 antigen)
Catalog Number: 14-1719
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio5G3 (5G3)
Isotype: Mouse IgG2a

Staining of Panc-1 cell line with 0.125 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (open histogram) or 0.125 μg of Purified anti-human CD171 (5G3) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1719	Affinity Purified anti-human CD171 (Neurite Cell Adhesion Molecule L1, L1 antigen)	N/A	N/A	FC IH/F IP WB
12-1719	Phycoerythrin (PE) anti-human CD171 (Neurite Cell Adhesion Molecule L1, L1 antigen)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody eBio5G3 recognizes CD171 also known as neural cell adhesion molecule L1. CD171 is a member of the Ig superfamily containing 6 extracellular Ig domains and five fibronectin type III-like repeats. CD171 has been shown to function as a cell adhesion molecule mediating homotypic and heterotypic cell-cell interactions in neuronal myelination, neurite outgrowth and regeneration. Expression of CD171 has been found on monocytes and mature monocytic-derived and follicular DCs, a minor subset of lymphocytes in addition to that found on neuronal tissue and some tumor cells lines. Expression of CD171 on tumors is thought to contribute to tumor progression. Epitope of eBio5G3 is in amino-terminal Ig-like domain.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio5G3 (5G3) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) under nonreducing conditions, and immunohistology staining of frozen tissue sections.

Applications Tested

This eBio5G3 (5G3) antibody has been tested by flow cytometric of tumor cell line Panc-1. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Balaian LB, Moehler T, Montgomery AM. The human neural cell adhesion molecule L1 functions as a costimulatory molecule in T cell activation. Eur J Immunol. 2000 Mar;30(3):938-43. (5G3, FA PubMed)

Pancook JD, Reisfeld RA, Varki N, Vitiello A, Fox RI, Montgomery AM. Expression and regulation of the neural cell adhesion molecule L1 on human cells of myelomonocytic and lymphoid origin. J Immunol. 1997 May 1;158(9):4413-21. (5G3, FC PubMed)

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