Product Information

Contents: Affinity Purified anti-mouse ALCAM (CD166)
Catalog Number: 14-1661
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioALC48
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with 0.125 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.125 μg of Purified anti-mouse ALCAM (eBioALC-48) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1661	Affinity Purified anti-mouse ALCAM (CD166)	N/A	N/A	FC IHC(Paraffin)
12-1661	Phycoerythrin (PE) anti-mouse ALCAM (CD166)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

Activated leukocyte cell adhesion molecule (ALCAM; CD166) is a 105 kD cell-surface adhesion molecule belonging to the Ig superfamily of receptors. ALCAM is a type I transmembrane glycoprotein, possessing five extracellular Ig domains, a transmembrane domain, and a short cytoplasmic tail. ALCAM is involved in homotypic adhesion, as well as heterotypic adhesion with its natural ligand, CD6. A soluble isoform of ALCAM has been shown to inhibit homophilic adhesive interactions. ALCAM is expressed on a variety of cell types, including activated T and B cells, monocyte-derived dendritic cells, and thymic epithelial cells. ALCAM expression has also been used as a marker for the isolation of pluripotent stem cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioALC-48 antibody has been reported for use in flow cytometric analysis and immunohistochemistry on paraffin-embedded tissue sections.

Applications Tested

The eBioALC-48 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 µg per million cells in a 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN, Figdor CG. Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells. Blood. 2006 Apr 15;107(8):3212-20. (PubMed)

Swart GW, Lunter PC, Kilsdonk JW, Kempen LC. Activated leukocyte cell adhesion molecule (ALCAM/CD166): signaling at the divide of melanoma cell clustering and cell migration? Cancer Metastasis Rev. 2005 Jun;24(2):223-36. (PubMed)

van Kempen LC, Nelissen JM, Degen WG, Torensma R, Weidle UH, Bloemers HP, Figdor CG, Swart GW. Molecular basis for the homophilic activated leukocyte cell adhesion molecule (ALCAM)-ALCAM interaction. J Biol Chem. 2001 Jul 13;276(28):25783-90. (PubMed)

Bowen MA, Aruffo AA, Bajorath J. Cell surface receptors and their ligands: in vitro analysis of CD6-CD166 interactions. Proteins. 2000 Aug 15;40(3):420-8. (PubMed)

Related Products

Cat. 14-4321 Affinity Purified Rat IgG2a Isotype Control