Product Information

Contents: Affinity Purified anti-human CD165 (AD2, gp37)
Catalog Number: 14-1659
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioSN2 (SN2 N6-D11)
Isotype: Mouse IgG1

Staining of Jurkat cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD165 (SN2) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1659	Affinity Purified anti-human CD165 (AD2, gp37)	N/A	N/A	FC IH/F IP
12-1659	Phycoerythrin (PE) anti-human CD165 (AD2, gp37)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioSN2 monoclonal antibody reacts with human CD165 (AD2, gp37). Human CD165 is a 37 kDa protein, which is thought to play a role in mediating adhesive interactions between thymocytes and epithelial cells in the thymus. CD165 was initially characterized as an antigenic marker of human T cell leukemias, and the SN2 monoclonal antibody was used to create immunotoxins capable of killing CD165-expressing cells. CD165 is expressed at low levels on all T cells, but at high levels on CD4-CD8- (double-negative) and CD4+CD8+ (double-positive) T cells in the thymus. Furthermore, CD165 is expressed by monocytes, platelets and mesothelial cells, and expression has been detected in islet cells of the pancreas, Bowman’s capsule of the kidney and, on central nervous system neurons. It is thought that CD165 and TALLA-1 represent the same antigen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioSN2 (SN2 N6-D11) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.

Applications Tested

This eBioSN2 (SN2 N6-D11) antibody has been tested by flow cytometric analysis of Jurkat cells and normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Negoro S, Seon BK. Several new monoclonal antibodies directed to human T-cell leukemia antigens. Cancer Res. 1982 Oct;42(10):4259-62. (SN2, mAb development, PubMed)

Seon BK. Specific killing of human T-leukemia cells by immunotoxins prepared with ricin A chain and monoclonal anti-human T-cell leukemia antibodies. Cancer Res. 1984 Jan;44(1):259-64. (SN2, PubMed)

Seon BK, Negoro S, Barcos MP, Tebbi CK, Chervinsky D, Fukukawa T. Monoclonal antibody SN2 defining a human T cell leukemia-associated cell surface glycoprotein. J Immunol. 1984 Apr;132(4):2089-95. (SN2, radioimmunoassay, IF, IP, PubMed)

Bruggers CS, Patel DD, Scearce RM, Whichard LP, Haynes BF, Singer KH. AD2, a human molecule involved in the interaction of T cells with epidermal keratinocytes and thymic epithelial cells. J Immunol. 1995 Mar 1;154(5):2012-22.

Takagi S, Fujikawa K, Imai T, Fukuhara N, Fukudome K, Minegishi M, Tsuchiya S, Konno T, Hinuma Y, Yoshie O. Identification of a highly specific surface marker of T-cell acute lymphoblastic leukemia and neuroblastoma as a new member of the transmembrane 4 superfamily. Int J Cancer. 1995 May 29;61(5):706-15. (SN2, FC, PubMed)

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