Staining of Jurkat cell line with 0.25 µg of Mouse IgG1 κ Isotype Control Purified (cat. 14-4714) (open histogram) or 0.25 µg of Anti-Human CD165 Purified (filled histogram) followed by Anti-Mouse IgG Biotin (cat. 13-4013) and Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Anti-Human CD165 Purified Catalog Number: 14-1659 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Clone: eBioSN2 (SN2 N6-D11) Host/Isotype: Mouse IgG1
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Size
Price
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14-1659-80
25 ug
14-1659-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBioSN2 monoclonal antibody reacts with human CD165 (AD2, gp37). Human CD165 is a 37 kDa protein, which is thought to play a role in mediating adhesive interactions between thymocytes and epithelial cells in the thymus. CD165 was initially characterized as an antigenic marker of human T cell leukemias, and the SN2 monoclonal antibody was used to create immunotoxins capable of killing CD165-expressing cells. CD165 is expressed at low levels on all T cells, but at high levels on CD4-CD8- (double-negative) and CD4+CD8+ (double-positive) T cells in the thymus. Furthermore, CD165 is expressed by monocytes, platelets and mesothelial cells, and expression has been detected in islet cells of the pancreas, Bowman’s capsule of the kidney and, on central nervous system neurons. It is thought that CD165 and TALLA-1 represent the same antigen.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioSN2 (SN2 N6-D11) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.
Applications Tested
This eBioSN2 (SN2 N6-D11) antibody has been tested by flow cytometric analysis of Jurkat cells and normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Negoro S, Seon BK.
Several new monoclonal antibodies directed to human T-cell leukemia antigens.
Cancer Res. 1982 Oct;42(10):4259-62. (SN2, mAb development, PubMed)
Seon BK.
Specific killing of human T-leukemia cells by immunotoxins prepared with ricin A chain and monoclonal anti-human T-cell leukemia antibodies.
Cancer Res. 1984 Jan;44(1):259-64. (SN2, PubMed)
Seon BK, Negoro S, Barcos MP, Tebbi CK, Chervinsky D, Fukukawa T.
Monoclonal antibody SN2 defining a human T cell leukemia-associated cell surface glycoprotein.
J Immunol. 1984 Apr;132(4):2089-95. (SN2, radioimmunoassay, IF, IP, PubMed)
Bruggers CS, Patel DD, Scearce RM, Whichard LP, Haynes BF, Singer KH.
AD2, a human molecule involved in the interaction of T cells with epidermal keratinocytes and thymic epithelial cells.
J Immunol. 1995 Mar 1;154(5):2012-22.
Takagi S, Fujikawa K, Imai T, Fukuhara N, Fukudome K, Minegishi M, Tsuchiya S, Konno T, Hinuma Y, Yoshie O.
Identification of a highly specific surface marker of T-cell acute lymphoblastic leukemia and neuroblastoma as a new member of the transmembrane 4 superfamily.
Int J Cancer. 1995 May 29;61(5):706-15. (SN2, FC, PubMed)
