Product Information

Contents: Affinity Purified anti-human CD163 (M130; Haemoglobin Scavenger Receptor, Macrophage marker)
Catalog Number: 14-1639
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioGHI/61 (GHI/61)
Isotype: Mouse IgG1, κ

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD163 (GHI/61) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1639	Affinity Purified anti-human CD163 (M130; Haemoglobin Scavenger Receptor, Macrophage marker)	N/A	N/A	FC WB
13-1639	Biotin anti-human CD163 (M130; Haemoglobin Scavenger Receptor, Macrophage marker)	N/A	N/A	FC
12-1639	Phycoerythrin (PE) anti-human CD163 (M130; Haemoglobin Scavenger Receptor, Macrophage marker)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family. CD163 is expressed by tissue macrophages, circulating blood monocytes, and some reports suggest that it may also be expressed by haematopoietic progenitor cells. CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin which forms to clear haemoglobin from the blood. The high affinity for CD163 is specific for the haemoglobin-haptoglobin complex, whereas haemoglobin or haptoglobin display no to low affinity toward CD163. Upon ligand binding, CD163 initiates signal transduction which leads to the production of IL-6 and IL-10. Furthermore, it has been demonstrated that cytokines such as IL-6 and IL-10 can induce expression of CD163, whereas proinflammatory stimuli, such as LPS or TNFα, can cause shedding of a soluble form of CD163, whose function is unclear.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioGHI/61 (GHI/61) antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB).

Applications Tested

This eBioGHI/61 (GHI/61) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Pulford K, Micklem K, McCarthy S, Cordell J, Jones M, Mason DY. A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4. Immunology. 1992 Apr;75(4):588-95. (GHI/61, FC, PubMed)

Law SK, Micklem KJ, Shaw JM, Zhang XP, Dong Y, Willis AC, Mason DY. A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. Eur J Immunol. 1993 Sep;23(9):2320-5. (GHI/61, FC, PubMed)

Hogger P, Dreier J, Droste A, Buck F, Sorg C. Identification of the integral membrane protein RM3/1 on human monocytes as a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family (CD163). J Immunol. 1998 Aug 15;161(4):1883-90.

Matthews KE, Mueller SG, Woods C, Bell DN. Expression of the hemoglobin-haptoglobin receptor CD163 on hematopoietic progenitors. Stem Cells Dev. 2006 Feb;15(1):40-8.

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