Product Information

Contents: Affinity Purified anti-human CD157 (BST-1, BP-3/IF-7)
Catalog Number: 14-1579
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioSY11B5 (SY11B5)
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD157 (SY11B5) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1579	Affinity Purified anti-human CD157 (BST-1, BP-3/IF-7)	N/A	N/A	FC IH/F IP WB
12-1579	Phycoerythrin (PE) anti-human CD157 (BST-1, BP-3/IF-7)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioSY11B5 monoclonal antibody recognizes human CD157 (Mo5, BST-1). CD157 is a 42-45 kDa, GPI-anchored protein with structural and functional similarities with CD38. CD157 was initially cloned because of its expression on monocytes and macrophages, and was subsequently discovered to be the same protein named BST-1, discovered for its expression on bone marrow stromal cells and its ability to stimulate the proliferation of a mouse pre-B cell line. CD157 is a pleiotropic ectoenzyme and is thought to act independently as an enzyme and receptor. Similar to CD38, CD157 is involved in the metabolism of NAD+ and this activity may be involved in regulating intracellular Ca2+ levels. As a receptor, upon binding of its putative ligand, CD157 is thought to initiate a signal transduction cascade resulting in the phosphorylation of cytoplasmic proteins including focal adhesion kinase (FAK). The mechanism and functional significance of CD157-initiated signal transduction remain to be fully characterized.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioSY11B5 (SY11B5) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) (non-reduced only), and immunohistology staining of frozen tissue sections.

Applications Tested

This eBioSY11B5 (SY11B5) antibody has been tested by flow cytometric analysis of normal human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ortolan E, Vacca P, Capobianco A, Armando E, Crivellin F, Horenstein A, Malavasi F. CD157, the Janus of CD38 but with a unique personality. Cell Biochem Funct. 2002 Dec;20(4):309-22. Review.

Kaisho T, Ishikawa J, Oritani K, Inazawa J, Tomizawa H, Muraoka O, Ochi T, Hirano T. BST-1, a surface molecule of bone marrow stromal cell lines that facilitates pre-B-cell growth. Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5325-9.

Muraoka O, Tanaka H, Itoh M, Ishihara K, Hirano T. Genomic structure of human BST-1. Immunol Lett. 1996 Dec 1;54(1):1-4.

Goldstein SC, Todd RF 3rd. Structural and biosynthetic features of the Mo5 human myeloid differentiation antigen. Tissue Antigens. 1993 Apr;41(4):214-8.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control